Highly selective resonance scattering detection of trace thrombin using aptamer-modified AuRe nanoprobe

Bioprocess and Biosystems Engineering
Aihui LiangZhi-Liang Jiang

Abstract

The gold-rhenium (AuRe) composite nanoparticle was prepared by NaBH(4) reduction procedure, and was modified by the aptamer to obtain an AuRe nanoprobe (AuRessDNA) for thrombin. In pH 7.0 Tris-HCl buffer solution and in the presence of salt, the nanoprobe specifically combined with thrombin to form AuRe-aptamer-thrombin cluster that resulted in the resonance scattering intensity (I (560 nm)) increasing at 560 nm. The increased intensity ΔI (560 nm) was linear to the thrombin concentration in the range of 0.115-6.93 nmol/L, with a regression equation of ΔI (560 nm) = 53.0 C + 2.5, a correlation coefficient of 0.9989, and a detection limit of 13 pmol/L. This method was applied to detect thrombin in human plasma samples, with satisfactory results.

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Citations

Nov 30, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Branislav Ruttkay-NedeckyVojtech Adam
May 1, 2012·Biosensors & Bioelectronics·Zhanguang ChenJinbin Liu
Jan 7, 2015·Chemical Society Reviews·Haitao MaYuan Wan
Sep 1, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Meiling TangZhiliang Jiang
Feb 7, 2012·Chemical Communications : Chem Comm·Xueyang ZhangXiangmin Zhang

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