Highly Sensitive and Practical Fluorescent Sandwich ELISA for Ciguatoxins

Analytical Chemistry
Takeshi TsumurayaIkuo Fujii

Abstract

Ciguatera fish poisoning (CFP) caused by the consumption of fish that have accumulated ciguatoxins (CTXs) affects more than 50000 people annually. The spread of CFP causes enormous damage to public health, fishery resources, and the economies of tropical and subtropical endemic regions. The difficulty in avoiding CFP arises from the lack of sensitive and reliable analytical methods for the detection and quantification of CTXs in contaminated fish, along with the normal appearance, smell, and taste of fish contaminated with the causative toxins. Thus, an accurate, sensitive, routine, and portable detection method for CTXs is urgently required. We have successfully developed a highly sensitive fluorescent sandwich ELISA, which can detect, differentiate, and quantify four major CTX congeners (CTX1B, CTX3C, 51-hydroxyCTX3C, and 54-deoxyCTX1B) with a detection limit of less than 1 pg/mL. The ELISA protocol, using one microtiter plate coated with two mAbs (10C9 and 3G8), and ALP-linked 8H4, can detect any of the four CTX congeners in a single operation. CTX1B spiked into fish at the FDA guidance level of 0.01 ppb CTX1B equivalent toxicity in fish from Pacific regions was also proven to be reliably detected by this ELISA. Furthermore,...Continue Reading

References

Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·R J LewisM M Sheil
May 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·M A PoliL G Carpenter
Jan 27, 1999·Toxicon : Official Journal of the International Society on Toxinology·M Y DechraouiA M Legrand
Aug 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·R J Lewis
Dec 1, 2001·Science·M HiramaM Satake
Mar 16, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·T Yasumoto
Aug 15, 2002·Toxicon : Official Journal of the International Society on Toxinology·Brett HamiltonRichard J Lewis
Sep 11, 2002·Toxicon : Official Journal of the International Society on Toxinology·Brett HamiltonRichard J Lewis
Jun 19, 2003·Journal of the American Chemical Society·Hiroki OguriYoko Nagumo
Jun 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Masayuki InoueMasahiro Hirama
Jul 15, 2004·Journal of Immunological Methods·Yoko NagumoIzumi Kumagai
Jul 20, 2006·Journal of the American Chemical Society·Masayuki InoueMasahiro Hirama
Aug 8, 2006·Toxicon : Official Journal of the International Society on Toxinology·Takeshi TsumurayaMasahiro Hirama
Oct 20, 2006·Angewandte Chemie·Anouk DirksenPhilip E Dawson
Mar 24, 2009·Toxicon : Official Journal of the International Society on Toxinology·Richard J LewisAlun Jones
Jun 16, 2009·Toxicon : Official Journal of the International Society on Toxinology·Takeshi TsumurayaMasahiro Hirama
Sep 29, 2009·Toxicon : Official Journal of the International Society on Toxinology·Robert W Dickey, Steven M Plakas
Jun 19, 2010·Analytical Chemistry·Paz OteroLuis M Botana
Jan 22, 2011·Journal of Natural Products·Shuji YamashitaMasahiro Hirama
May 12, 2012·Toxicon : Official Journal of the International Society on Toxinology·Takeshi TsumurayaMasahiro Hirama
Jan 7, 2014·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Kentaro YogiTakeshi Yasumoto
Apr 14, 2016·PloS One·D Ransom HardisonR Wayne Litaker

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Citations

Jul 30, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ruru ChenBing Wang
Dec 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yue ZhangYanfei Shen
Aug 6, 2020·Toxins·Tibor PasinszkiTodd E Dennis
Apr 4, 2021·International Journal of Environmental Research and Public Health·Christopher R LoefflerDorina Bodi
Aug 10, 2020·Ecotoxicology and Environmental Safety·G GaianiM Campàs

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