Highly sensitive and selective detection of Hg2+ using mismatched DNA and a molecular light switch complex in aqueous solution

Biosensors & Bioelectronics
Xiaoru ZhangShusheng Zhang

Abstract

A novel "signal-on" assay for sensitive and selective detection of Hg(2+) in aqueous solution based on mismatched DNA and a molecular light switch complex, [Ru(phen)(2)(dppz)](2+), was reported in the present work. This Hg(2+) specific sensor system composed of two label-free DNA probes with eight T-T mismatches, which could form stable DNA duplexes by thymine-Hg(2+)-thymine (T-Hg(2+)-T) in the presence of Hg(2+). The luminescence of [Ru(phen)(2)(dppz)](2+) is very weak in aqueous solution, and significant luminescence can be observed when intercalating into DNA duplexes. By monitoring Hg(2+)-dependent luminescence intensity enhancement, highly sensitive and selective determination of Hg(2+) has been achieved. Under the optimum conditions, the luminescence intensity was proportional to the concentration of Hg(2+) in the range of 1.0x10(-9)-1.5x10(-7) M. A detection limit of 3.5x10(-10) M Hg(2+) was achieved using 3sigma. The presence of other metal ions did not interfere with the detection of Hg(2+) even at high concentrations. This approach is simple and rapid, which can be used to monitor the Hg(2+) concentration in drinking water and natural media within 30 min.

Citations

Mar 5, 2011·Annual Review of Analytical Chemistry·Xiao-Bing ZhangYi Lu
Aug 15, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuyu ZhangHui Wei
Jul 6, 2014·Luminescence : the Journal of Biological and Chemical Luminescence·Meiling TangZhiliang Jiang
Feb 20, 2015·Analytical and Bioanalytical Chemistry·Zhanming LiTao Wu
Jul 19, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Guanying LiHui Chao
Oct 31, 2014·The Analyst·Guichi Zhu, Chun-yang Zhang
Jan 26, 2013·Chemical Society Reviews·Dik-Lung MaChung-Hang Leung

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