Abstract
Dermatophagoides farinae (Der f) is a major allergen in Der f extract. Measurement of Der f I-specific IgE has not been commonly used, because of the difficulty in obtaining large amounts of purified Der f 1. To improve the diagnosis of dust mite allergy, we wanted to develop an ELISA to measure Der f 1-specific IgE in which purified Der f 1 is not required. Using a monoclonal antibody (mAb) against Der f 1, a mAb-capture ELISA was developed. Microplates coated with the mAb were sequentially incubated with crude Der f extract, serum samples, goat anti-human IgE, and conjugated rabbit anti-goat IgG. A conventional ELISA using purified Der f 1 as capture (Der f 1-ELISA) was developed to compare it with the mAb-capture ELISA. There was a significant correlation between the two ELISAs (r = 0.89, P < .001) with a sensitivity of 0.8 U/mL. Both the assays were inhibited by the crude Der f extract in a dose-dependent manner. Using the two ELISAs, serum Der f 1-specific IgE was evaluated in 12 allergic patients with positive skin tests to Der f extract, 14 allergic patients with negative skin tests to Der f extract, and 15 healthy subjects. The mean Der f 1-IgE and the positivity rate of Der f I-IgE were higher in the mite-allergic grou...Continue Reading
References
Oct 1, 1992·Allergy·T NaganoY Masuda
May 1, 1992·The Journal of Allergy and Clinical Immunology·T A Platts-MillsM D Champman
Apr 1, 1992·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H D ShenS H Han
Dec 15, 1991·Journal of Immunological Methods·A Didierlaurent, M Garcelon
Feb 1, 1991·The Journal of Allergy and Clinical Immunology·S M PollartM D Chapman
Jun 1, 1990·The Journal of Allergy and Clinical Immunology·J BousquetF B Michel
Jan 1, 1990·Allergy·P W Ewan, D Coote
May 1, 1988·The Journal of Allergy and Clinical Immunology·J S van der ZeeR C Aalberse
Nov 1, 1988·The Journal of Allergy and Clinical Immunology·H de GrootR C Aalberse
May 10, 1985·Journal of Immunological Methods·R UrbanekD Samuel
Jan 1, 1988·Allergy·M D Chapman
Aug 1, 1987·The Journal of Allergy and Clinical Immunology·M D ChapmanT A Platts-Mills
Apr 22, 1985·Journal of Immunological Methods·D M KemenyD Richards
Feb 27, 1986·Journal of Immunological Methods·D M KemenyH J Maasch
Sep 1, 1971·Immunochemistry·E Engvall, P Perlmann
Jun 1, 1983·Clinical Rheumatology·T NishimakiR Kasukawa
Aug 1, 1994·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·B FahlbuschL Jäger
Sep 1, 1994·International Archives of Allergy and Immunology·A M WittemanR C Aalberse
Oct 1, 1993·The Journal of Allergy and Clinical Immunology·S H HanM F Tam
Oct 1, 1995·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R AyusoF Polo
Jul 1, 1995·Allergy·L J LuJ N Fink
Citations
Oct 21, 1999·Insect Biochemistry and Molecular Biology·Z PengF E Simons
May 31, 2001·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·D A SilvaE A Taketomi
Jan 1, 2011·ISRN Allergy·Dhruba SharmaA B Singh