Highly sensitive and specific high-performance liquid chromatographic analysis of 7-hydroxywarfarin, a marker for human cytochrome P-4502C9 activity

Journal of Chromatography. B, Biomedical Applications
D Lang, R Böcker

Abstract

The formation of 7-hydroxywarfarin in incubations of (S)-warfarin with human liver microsomes reflects their cytochrome P-4502C9 activity. This paper describes a rapid high-performance liquid chromatographic method for the determination of 7-hydroxywarfarin with high sensitivity, selectivity, and a simple sample clean-up procedure. Separation was achieved with a C18 reversed-phase column and quantification by fluorometric detection. The method employs an internal standard resulting in good accuracy and precision. The limit of detection is 150 fmol for 7-hydroxywarfarin.

Citations

Mar 17, 2004·Toxicology Letters·Shuji OhhiraHisao Matsui
Apr 21, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Charles W LocusonTimothy S Tracy
Jan 28, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Mingli YangNianqiang Wu
Dec 26, 2002·Japanese Journal of Pharmacology·Keizo UmegakiKazumasa Shinozuka
Jun 12, 2013·The Pharmacogenomics Journal·Y NiinumaM Hiratsuka
Oct 25, 2002·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·N HagenS H Hansen
Jan 16, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S E Clarke
May 12, 2009·Drug Metabolism and Pharmacokinetics·Tae InoueShigeru Ohta
Apr 13, 2011·Expert Opinion on Drug Metabolism & Toxicology·Drew R Jones, Grover P Miller
Mar 1, 2006·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·W A DanielJ Boksa
May 21, 2016·Frontiers in Pharmacology·Huihui DuShengying Qin
Jan 6, 2018·Reports on Progress in Physics·Christopher D BostickDavid Lederman
Feb 7, 2019·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Huanying YeYuan Wei
Jan 24, 2006·American Journal of Therapeutics·Michael A MohutskyGary W Elmer
May 4, 2018·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A V KuzikovV V Shumyantseva
Aug 6, 2014·Current Hypertension Reports·Idris GuessousMurielle Bochud
Jul 1, 1999·Alternatives to Laboratory Animals : ATLA·S CoeckeC Westmoreland
Sep 12, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Tae InoueShigeru Ohta
Mar 27, 2004·Molecular Pharmacology·Leslie J DickmannAllan E Rettie
Nov 16, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Rae YuanShiew-Mei Huang
Apr 21, 2001·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·C EmotoT Yokoi
Aug 25, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Laura TurcoAnnalaura Stammati

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