Highly-sensitive aptasensor based on fluorescence resonance energy transfer between l-cysteine capped ZnS quantum dots and graphene oxide sheets for the determination of edifenphos fungicide

Biosensors & Bioelectronics
Majid Arvand, Aazam A Mirroshandel

Abstract

With the advantages of excellent optical properties and biocompatibility, single-strand DNA-functionalized quantum dots have been widely applied in biosensing and bioimaging. A new aptasensor with easy operation, high sensitivity, and high selectivity was developed by immobilizing the aptamer on water soluble l-cysteine capped ZnS quantum dots (QDs). Graphene oxide (GO) sheets are mixed with the aptamer-QDs. Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QDs ensemble. This aptasensor enables the energy transfer based on a fluorescence resonance energy transfer (FRET) from the QDs to the GO sheets, quenching the fluorescence of QDs. The GO/aptamer-QDs ensemble assay acts as a "turn-on'' fluorescent sensor for edifenphos (EDI) detection. When GO was replaced by EDI, the fluorescence of QDs was restored and its intensity was proportional to the EDI concentration. This GO-based aptasensor under the optimum conditions exhibited excellent analytical performance for EDI determination, ranging from 5×10-4to 6×10-3mg L-1with the detection limit of 1.3×10-4mgL-1. Furthermore, the designed aptasensor exhibited excellent selectivity toward EDI compared to other pesticides and herbicides with similar str...Continue Reading

Citations

Sep 23, 2020·Journal of Molecular Modeling·Jingyu ZhangLiang Wu
Jul 25, 2019·Mikrochimica Acta·Zeki Semih PehlivanMeral Yüce
Sep 8, 2018·Foods·Denise CapoferriDario Compagnone
Dec 3, 2020·Sensors·Kamonrat Phopin, Tanawut Tantimongcolwat
Feb 10, 2019·Biosensors & Bioelectronics·Mei LiuNongyue He
Mar 26, 2021·ACS Applied Materials & Interfaces·Sifan DuMinghua Liu
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Weixiang LiuPengcheng Li

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