Highly sensitive cell imaging "Off-On" fluorescent probe for mitochondria and ATP

Biosensors & Bioelectronics
Priyanka SrivastavaArvind Misra

Abstract

A smart Off-On molecular scaffold/fluorescent probe 1 has been designed and synthesized. The probe has shown considerable photostability, cell permeability, organelle specificity and selectivity for ATP. The multicolor live cell imaging experiments in HeLa cells showed high selectivity of probe 1 for mitochondria with fluorescence "turn-on" response. As a proof of concept and promising prospects for application in biological sciences probe 1 has been utilized to detect ATP sensitively in a partial aqueous medium and intracellularly in HeLa cells. The favorable interaction between triphosphate unit of ATP and piperazine N atoms of probe 1 is attributed to synergistic effects of H-bonding and electrostatic interactions that encouraged the CH-π and π→π stacking between anthracene and purine rings. Consequently, the observed enhanced "turn-on" emission and a naked-eye sensitive blue-green color in the medium is attributable to arrest in photoinduced electron transfer (PET) process.

References

Mar 5, 1999·Science·D C Wallace

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Citations

Apr 4, 2017·Chemical Communications : Chem Comm·Jiang-Hua ZhuJong Seung Kim
Jul 15, 2017·Chemistry : a European Journal·Vasyl G PivovarenkoYves Mély
Oct 6, 2018·Chemistry : a European Journal·Aleksandr M AgafontsevEvgeny A Kataev
Apr 30, 2021·Biomedical Materials·Shanni HongYi Lu
Apr 10, 2020·ACS Applied Materials & Interfaces·Xin-Yao WangXue-Bo Yin
Jan 19, 2021·ACS Biomaterials Science & Engineering·Xiao Li JiangXiao Yong Tong
Nov 19, 2021·Chemical Society Reviews·Wenhui JiWei Huang

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