Highly Sensitive Detection of Chemically Modified Thio-Organophosphates by an Enzymatic Biosensing Device: An Automated Robotic Approach

Sensors
Giovanni Paolo CetrangoloFerdinando Febbraio

Abstract

Pesticides represent some of the most common man-made chemicals in the world. Despite their unquestionable utility in the agricultural field and in the prevention of pest infestation in public areas of cities, pesticides and their biotransformation products are toxic to the environment and hazardous to human health. Esterase-based biosensors represent a viable alternative to the expensive and time-consuming systems currently used for their detection. In this work, we used the esterase-2 from Alicyclobacillus acidocaldarius as bioreceptor for a biosensing device based on an automated robotic approach. Coupling the robotic system with a fluorescence inhibition assay, in only 30 s of enzymatic assay, we accomplished the detection limit of 10 pmol for 11 chemically oxidized thio-organophosphates in solution. In addition, we observed differences in the shape of the inhibition curves determined measuring the decrease of esterase-2 residual activity over time. These differences could be used for the characterization and identification of thio-organophosphate pesticides, leading to a pseudo fingerprinting for each of these compounds. This research represents a starting point to develop technologies for automated screening of toxic comp...Continue Reading

References

Jan 1, 1979·Archives of Oto-rhino-laryngology·S K Juhn, W D Ward
Jul 1, 1990·Environmental Health Perspectives·T R Fukuto
Nov 18, 2003·The Journal of Biological Chemistry·Giuseppina De SimoneGiuseppe Manco
Mar 31, 2005·Biosensors & Bioelectronics·Sofia SotiropoulouNikos A Chaniotakis
Jul 3, 2008·Journal of Agricultural and Food Chemistry·Matt J Hengel, Marion Miller
Jul 16, 2008·Extremophiles : Life Under Extreme Conditions·Ferdinando FebbraioGiuseppe Manco
Oct 24, 2008·Environmental Health : a Global Access Science Source·Marina Bjørling-PoulsenPhilippe Grandjean
Jun 11, 2009·Protein and Peptide Letters·Giuseppe MancoFerdinando Febbraio
Apr 22, 2011·Environmental Health Perspectives·Virginia RauhRobin Whyatt
Jan 10, 2012·Biosensors & Bioelectronics·Rupesh K MishraJean-Louis Marty
Jan 25, 2012·Neurotoxicology·Leslie LondonLorann Stallones
Jul 5, 2012·Analytical Biochemistry·Chandra Shekhar Pundir, Nidhi Chauhan
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Chang-jun HouCai-hong Shen
Jun 18, 2014·Environment International·Melissa A FurlongMary S Wolff
Oct 11, 2015·Analytical Biochemistry·Wirongrong WhangsukSuvit Loprasert
Oct 21, 2016·Archives of Toxicology·Shokoufeh HassaniMohammad Abdollahi
Mar 16, 2017·World Journal of Biological Chemistry·Ferdinando Febbraio
Jun 14, 2018·Chembiochem : a European Journal of Chemical Biology·Giorgio CasertaAngela Lombardi

❮ Previous
Next ❯

Citations

Aug 6, 2020·Sensors·Heba A HusseinFerdinando Febbraio
Jul 31, 2021·Journal of Hazardous Materials·Meng Shien GohNyuk Ling Ma
Sep 7, 2021·Analytical Methods : Advancing Methods and Applications·Monika BhattuDeepika Kathuria

❮ Previous
Next ❯

Methods Mentioned

BETA
biosensors
biosensor
Assay
chemical modification
biosensing

Software Mentioned

QtiPlot
® Star Vector
Microlab

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.