Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design

The Analyst
A Rahim RuslindaHiroshi Kawarada

Abstract

Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich design approach significantly increases the fluorescence signal intensity, and thereby a very low detection limit of 4 pM was achieved. The effect of the ionic strength of MgCl(2) buffer solution was also investigated, and the most favourable binding for PDGF-BB occurred at a Mg(2+) concentration of 5.5 mM. Since the aptamers bind to the target PDGF with high affinity, fluorescence detection exhibited high selectivity towards different biomolecules. The high reproducibility of detection was confirmed by performing three cycles of measurements over a period of three days.

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Citations

Jul 6, 2014·Analytical Chemistry·Chia-Wei Wang, Huan-Tsung Chang
Oct 21, 2015·Biosensors & Bioelectronics·Mengmeng YanAiliang Chen
May 15, 2013·Journal of Pharmaceutical and Biomedical Analysis·Tina ŠmucHenning Ulrich
Jul 31, 2012·Biosensors & Bioelectronics·Varun PenmatsaChunlei Wang
Nov 21, 2015·Analytical and Bioanalytical Chemistry·Miriam Jauset RubioCiara K O'Sullivan
Sep 15, 2012·Biosensors & Bioelectronics·A Rahim RuslindaHiroshi Kawarada
Jul 30, 2015·Analytical and Bioanalytical Chemistry·Andreas NaglRomana Schirhagl
Apr 29, 2015·Biosensors & Bioelectronics·Ailiang Chen, Shuming Yang
Apr 28, 2016·Scientific Reports·Eunseop YeomSang Joon Lee
Jul 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jun Seop LeeJyongsik Jang
Apr 21, 2021·Expert Review of Molecular Diagnostics·Rajesh AhirwarSaroj Kumar
Apr 22, 2020·ACS Applied Materials & Interfaces·Wooyoung KimJyongsik Jang

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