Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay

Nanoscale
Xiuli FuLingxin Chen

Abstract

Accurate analysis of prostate cancer specific biomarkers plays an important role in the early diagnosis of prostate cancer. Traditional colorimetric lateral flow assay (LFA) has the limitations of low detection sensitivity and qualitative or semiquantitative detection. In this study, we developed a novel surface-enhanced Raman scattering (SERS)-based competitive LFA for the rapid and highly sensitive quantitative evaluation of prostate cancer antigen 3 (PCA3) mimic DNA. Herein, the competitive hybridization interaction with capture DNA between target PCA3 mimic DNA and reporter DNA-labeled SERS nanotags results in a change in the amount of SERS nanotags on the test line. The quantitative analysis of target PCA3 mimic DNA was realized by monitoring the Raman peak intensity of SERS nanotags on the test line. The limit of detection of PCA3 mimic DNA was estimated to be 3 fM, which is about three orders of magnitude more sensitive than that of a commercially available kit. By combining the outstanding characteristics of the well-established SERS-based competitive strategy and LFA platform, our design has strong potential for the early diagnosis of prostate cancer and other diseases.

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Citations

Oct 22, 2019·Chembiochem : a European Journal of Chemical Biology·Sandeep KaushalDong Kee Yi
Mar 28, 2020·International Journal of Molecular Sciences·Maria Blanco-Formoso, Ramon A Alvarez-Puebla
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yogesh Kumar, Kairam Narsaiah
Nov 28, 2020·Klinicheskaia laboratornaia diagnostika·Boris Georgievich AndryukovE V Matosova
Mar 26, 2021·Analytical Chemistry·Yu-Hsuan ChenKui-Thong Tan
May 28, 2021·Expert Review of Molecular Diagnostics·Vincenza ConteducaUgo De Giorgi
May 30, 2021·Biosensors & Bioelectronics·Luyang WangLingxin Chen
Aug 7, 2021·Journal of Personalized Medicine·James MeehanArran K Turnbull
Nov 26, 2019·ACS Applied Materials & Interfaces·Juliana Coatrini SoaresOsvaldo N Oliveira

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Methods Mentioned

BETA
biopsy
PCR
biosensor
Raman scattering
ELISA
DNA assay
Dynamic light scattering
PCA
fluorescence assay
Assay

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