Highly sensitive electrochemical stripping detection of hepatitis B surface antigen based on copper-enhanced gold nanoparticle tags and magnetic nanoparticles

Analytica Chimica Acta
Guangyu Shen, Yun Zhang

Abstract

On the basis of copper-enhanced gold nanoparticle tags as an amplification approach, we introduced, in this paper, magnetic nanoparticles for further improving performance of electrochemical immunoassay by anodic stripping voltammetry (ASV) at a glassy-carbon electrode. Due to the use of antibody-immobilized magnetic nanoparticles, the immunoreaction between antibody and antigen takes place in a homogeneous bulk solution phase. Compared with traditional solid interface reaction, the proposed strategy can provide some advantages such as easy of separation, shorter analytical time, wider linear range, and lower detection limit. It was also successfully applied to HBsAg determination in a linear range of 0.1-1500 ng mL(-1) with a detection limit of 87 pg mL(-1). The proposed analytical strategy holds good selectivity, sensitivity and repeatability and also great promise for the extended application in the fields of clinical diagnosis, bio-affinity assay and environmental monitoring.

Citations

Feb 3, 2012·Chemical Reviews·Krishnendu SahaVincent M Rotello
Jun 28, 2011·Analytical Biochemistry·Xiaodong CaoSongqin Liu
Oct 7, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Hassan M E AzzazyRicardo Franco
Jul 9, 2013·Analytical Biochemistry·Sara NouraniSeyed Mehdi Boutorabi
Feb 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zeying ZhangYingshuai Liu
Sep 8, 2011·Journal of Environmental Monitoring : JEM·Chinnasamy ThiruppathirajaMuthukaruppan Alagar
Jul 6, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Muhammad RezkiBrian Yuliarto

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