Highly sensitive label-free bio-interfacial colorimetric sensor based on silk fibroin-gold nanocomposite for facile detection of chlorpyrifos pesticide.

Scientific Reports
Pramod ManeRavindra Chaudhari

Abstract

Herein, the preparation of gold nanoparticles-silk fibroin (SF-AuNPs) dispersion and its label-free colorimetric detection of the organophosphate pesticide, namely chlorpyrifos, at ppb level are reported. The silk fibroin solution was extracted from B. mori silk after performing degumming, dissolving and dialysis steps. This fibroin solution was used for synthesis of gold nanoparticles in-situ without using any external reducing and capping agent. X-ray Diffractometry (XRD), Field Emission Transmission Electron Microscopy (FETEM) along with Surface Plasmon Resonance based optical evaluation confirmed generation of gold nanoparticles within SF matrix. The resultant SF-AuNPs dispersion exhibited rapid and excellent colorimetric pesticide sensing response even at 10 ppb concentration. Effect of additional parameters viz. pH, ionic concentration and interference from other pesticide samples was also studied. Notably, SF-AuNPs dispersion exhibited selective colorimetric pesticide sensing response which can be calibrated. Furthermore, this method was extended to various simulated real life samples such as tap water, soil and agricultural products including plant residues to successfully detect the presence of chlorpyrifos pesticide. ...Continue Reading

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Citations

Mar 3, 2021·Analytica Chimica Acta·Patricia Ramirez-PriegoLaura M Lechuga
Apr 17, 2021·ACS Biomaterials Science & Engineering·Niranjana Jaya PrakashBalasubramanian Kandasubramanian
Jun 8, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jiajia YangJiping Chen
Sep 7, 2021·Analytical Methods : Advancing Methods and Applications·Monika BhattuDeepika Kathuria

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Methods Mentioned

BETA
electrophoresis
biosensors
biosensor
X-ray
Surface Plasmon Resonance

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