Highly sensitive label-free in vitro detection of aflatoxin B1 in an aptamer assay using optical planar waveguide operating as a polarization interferometer

Analytical and Bioanalytical Chemistry
Ali Al-JawdahAndrás Székács

Abstract

This work reports on further development of an optical biosensor for the in vitro detection of mycotoxins (in particular, aflatoxin B1) using a highly sensitive planar waveguide transducer in combination with a highly specific aptamer bioreceptor. This sensor is built on a SiO2-Si3N4-SiO2 optical planar waveguide (OPW) operating as a polarization interferometer (PI), which detects a phase shift between p- and s-components of polarized light propagating through the waveguide caused by the molecular adsorption. The refractive index sensitivity (RIS) of the recently upgraded PI experimental setup has been improved and reached values of around 9600 rad per refractive index unity (RIU), the highest RIS values reported, which enables the detection of low molecular weight analytes such as mycotoxins in very low concentrations. The biosensing tests yielded remarkable results for the detection of aflatoxin B1 in a wide range of concentrations from 1 pg/mL to 1 μg/mL in direct assay with specific DNA-based aptamers. Graphical abstract Optical planar waveguide polarization interferometry biosensor for detection of aflatoxin B1 using specific aptamer.

References

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Mar 2, 2016·Sensors·Adrián Fernández GavelaLaura M Lechuga
Jul 5, 2018·Toxins·Ali Al-JawdahAndras Szekacs

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Citations

Jan 5, 2020·Analytical and Bioanalytical Chemistry·Hongmei LiuYunxia Luan
Jun 12, 2021·ACS Sensors·Mohamed A EttabibJames S Wilkinson

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Methods Mentioned

BETA
biosensing
biosensors
biosensor
chip
PCR
chips

Software Mentioned

SPLICCO
Origin
TIRE

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