Highly sensitive oligonucleotide-based fluorometric detection of mercury(II) in aqueous media

Biosensors & Bioelectronics
Bin Liu

Abstract

This paper describes a highly sensitive and selective Hg(2+) sensor using a label free Hg(2+) specific probe (5'-18T-3') and an intercalation dye SYBR Green I (SG). The Hg(2+) specific probe is composed of thymines (T) and readily forms T-Hg(2+)-T complexes in the presence of Hg(2+). This specific T-Hg(2+)-T formation affects the hybridization of the Hg(2+) specific probe and the intercalation of SG. Upon treatment of 1 nM 5'-18T-3' with different amount of Hg(2+) (0.1-10nM), which is followed by hybridization with 1 nM 5'-18T-3' and incubation with 1 microL of SG, the solution fluorescence gave a linear response (R=0.996) to the concentration of Hg(2+). The detection limit for Hg(2+) was 0.5 nM (0.1 ppb). The overall test only takes few minutes and very little interference is observed from non-specific metal ions. This approach may find potential applications in monitoring the Hg(2+) concentration in drinking water.

Citations

Mar 5, 2011·Annual Review of Analytical Chemistry·Xiao-Bing ZhangYi Lu
Sep 12, 2015·Biosensors & Bioelectronics·Lanying LiGang Liu
Feb 26, 2016·Chemical Communications : Chem Comm·Ki Soo ParkHyun Gyu Park
Oct 31, 2014·The Analyst·Guichi Zhu, Chun-yang Zhang
Jan 26, 2013·Chemical Society Reviews·Dik-Lung MaChung-Hang Leung
Feb 22, 2011·Chemistry, an Asian Journal·Dik-Lung MaChung-Hang Leung
Dec 12, 2017·Inorganic Chemistry·Guochen BaoKa-Leung Wong

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