Highly sensitive optical fibre long period grating biosensor anchored with silica core gold shell nanoparticles

Biosensors & Bioelectronics
L MarquesSergiy Korposh

Abstract

An optical fibre long period grating (LPG), modified with a coating of silica core gold shell (SiO2:Au) nanoparticles (NPs) deposited using the layer-by-layer method, was employed for the development of a biosensor. The SiO2:Au NPs were electrostatically assembled onto the LPG with the aid of a poly(allylamine hydrochloride) (PAH) polycation layer. The LPG sensor operates at the phase matching turning point to provide the highest sensitivity. The SiO2:Au NPs were modified with biotin, which was used as a ligand for streptavidin (SV) detection. The sensing mechanism is based on the measurement of the refractive index change induced by the binding of the SV to the biotin. The effect on sensitivity of increasing the surface area by virtue of the SiO2:Au nanoparticles' diameter and film thickness was studied. The lowest measured concentration of SV was 2.5nM, achieved using an LPG modified with a 3 layer (PAH/SiO2:Au) thin film composed of SiO2 NPs of 300nm diameter with a binding constant of k=1.7(pM)(-1), sensitivity of 6.9nm/ng/mm(2) and limit of detection of 19pg/mm(2).

References

Dec 19, 2002·Molecular & Cellular Proteomics : MCP·N Leigh Anderson, Norman G Anderson
Apr 3, 2003·Nature Reviews. Cancer·Julia D WulfkuhleEmanuel F Petricoin
Apr 3, 2008·Optics Letters·Carlos A BarriosR Casquel
Jul 8, 2009·Optics Express·Séverine MaguisMarie-Claude Millot

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Citations

Dec 1, 2017·Sensors·Tuan GuoChristophe Caucheteur
Feb 10, 2019·Sensors·Pedro J RiveroFrancisco J Arregui
Jan 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Deependra TyagiZhijun Yang

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