Highly sensitive piezoelectric immunosensors employing signal amplification with gold nanoparticles.

Nanotechnology
Jiwon Kwak, Soo Suk Lee

Abstract

We present a quartz crystal microbalance (QCM) immunosensor for highly sensitive detection of prostate-specific antigen (PSA) in a human serum immunoassay. In particular, in this study, we employed signal amplification using and enlarging gold nanoparticles. Because QCM measures the change of resonance frequency according to the mass change occurring on the sensor surface, we could quantitatively analyze PSA based on a tremendous increase in mass by sandwich immunoassay using AuNP-conjugated anti-PSA-detecting antibody enhanced with subsequent gold staining. The limit of detection of the PSA immunoassay in human serum without gold staining enhancement was 687 pg ml-1 but was 48 pg ml-1 with the gold staining-mediated signal amplification. That is, amplifying the signal resulted in increased sensitivity and reproducibility of immunoassay in a human serum.

References

Apr 25, 1991·The New England Journal of Medicine·W J CatalonaG L Andriole
Jun 1, 1989·Analytical Biochemistry·P D MizeT H Schulte
Sep 8, 2000·Science·T A TatonR L Letsinger
Dec 18, 2002·Biosensors & Bioelectronics·Ilaria MannelliMarco Mascini
Feb 26, 2004·Journal of Immunological Methods·Ru-Qiang LiangKang-Cheng Ruan
Feb 11, 2005·Journal of the American Chemical Society·Nathan C GianneschiChad A Mirkin
Mar 28, 2007·Chemical Reviews·Samuel W ThomasTimothy M Swager
Apr 14, 2007·Analytical Chemistry·Shalini GuptaOrlin D Velev
Aug 30, 2008·Nature Methods·Ute Resch-GengerThomas Nann
Oct 8, 2008·Biosensors & Bioelectronics·Chia-Hsien YehYu-Cheng Lin
Nov 17, 2009·Advanced Drug Delivery Reviews·Sarit S AgastiVincent M Rotello
May 18, 2011·Chemical Society Reviews·Paolo Scrimin, Leonard J Prins
Jun 28, 2011·Analytical Biochemistry·Xiaodong CaoSongqin Liu
Sep 10, 2011·Chemistry : a European Journal·Rotem Perry-FeigenbaumDoron Shabat
Dec 8, 2011·IEEE Pulse·Elain FuJohn T McDevitt
Oct 10, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Edina C Wang, Andrew Z Wang
Jun 28, 2014·Biomolecules·Olga V Makhlynets, Ivan V Korendovych
Jan 15, 2015·Chemical Communications : Chem Comm·Jing ZhuWei Jiang
Dec 23, 2015·Sensors·Gonca BülbülSilvana Andreescu
Oct 6, 2016·Journal of the American Chemical Society·Xiaolong YangFeng Li
Nov 24, 2016·Diagnostics·Mílton CordeiroPedro Viana Baptista
Jul 29, 2017·Chemical Reviews·Zdeněk FarkaPetr Skládal
Jul 17, 2014·Materials·Yuanchao ZhangWenrong Yang
Nov 29, 2017·Journal of the American Chemical Society·Adam M KabzaJonathan T Sczepanski
Dec 7, 2017·Chemistry : a European Journal·Jie WangMingdong Dong
Dec 7, 2017·Nanoscale·H AldewachiP Gardiner
Apr 28, 2018·Nanomaterials·Zeyang Li, Guan-Yu Chen
Dec 2, 2016·Light, Science & Applications·Yu-Chao LiBao-Jun Li
Dec 12, 2018·ACS Applied Materials & Interfaces·Jie WangMingdong Dong
Mar 2, 2019·Nanomaterials·Lin LiuNing Xia

❮ Previous
Next ❯

Citations

Jan 30, 2020·Nanomaterials·Mingfei PanShuo Wang
Oct 15, 2020·Analytical Methods : Advancing Methods and Applications·Ji Yoon Lim, Soo Suk Lee
May 18, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Ningning YueAiping Fan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.