Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging

Chemical Science
Hua LiXianzhong Zhang

Abstract

A novel Förster resonance energy transfer (FRET) fluorescence "off-on" system based on the highly specific, sensitive and effective C-C bond cleavage of certain dihydropyridine derivatives was reported for real-time quantitative imaging of nitric oxide (NO). 1,4-Dihydropyridine was synthesized as a novel linker which could connect customized fluorophores and their corresponding quenchers. The specific and quantitative response to NO is confirmed using fluorescence spectrometry with the classical example of fluorescein isothiocyanate (FITC) and [4'-(N,N'-dimethylamino)phenylazo] benzoyl (DABCYL). The fluorescence intensity increased linearly with the increase in the amount of NO. Cells incubated with an exogenous NO donor emitted fluorescence as expected. A high fluorescence intensity was detected in macrophages which generate NO when incubated with lipopolysaccharide (LPS). The in vivo imaging shows about an 8-fold contrast between Freund's adjuvant stimulated feet and normal feet in mice after intravenous injection, which was the first example of in vivo semiquantitative fluorescence imaging of NO in mammals.

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroG Chaudhuri
Sep 29, 2001·Nature Immunology·C Bogdan
Apr 11, 2002·Chemical Reviews·Tetsuo Nagano, Tetsuhiko Yoshimura
Jun 10, 2004·Chemical Reviews·Scott R TrenorTimothy E Long
Sep 21, 2004·Nature Reviews. Microbiology·Ferric C Fang
Mar 18, 2005·Journal of the American Chemical Society·Eita SasakiTetsuo Nagano
Jul 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Koustubh PandaDennis J Stuehr
Dec 23, 2006·Antioxidants & Redox Signaling·Masao KanekiKyungho Chang
Jul 21, 2007·Free Radical Biology & Medicine·Sandrine LepillerJohanna Chluba
Nov 30, 2007·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Guanshu LiuMark D Pagel
Apr 16, 2008·Journal of Cell Science·Mi-Yeon KimHee-Sae Park
Sep 17, 2008·Angewandte Chemie·Xiaolin ZhangXuhong Qian
Sep 23, 2008·Free Radical Biology & Medicine·Jie OuyangJunfeng Zhang
Jul 16, 2009·Nitric Oxide : Biology and Chemistry·Catherine N Hall, John Garthwaite
Aug 3, 2010·Journal of the American Chemical Society·Youjun YangJason B Shear
May 7, 2011·Chemical Communications : Chem Comm·Yonggang ChenJingli Yuan
May 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ciro ColettaCsaba Szabo
Jun 30, 2012·Nitric Oxide : Biology and Chemistry·Yan-Fei MaoJun-Long Huang
Aug 22, 2012·Chemistry : a European Journal·Eun Won SeoBong Rae Cho
May 31, 2013·Neuron·Tomohiro NakamuraStuart A Lipton
Mar 13, 2014·Journal of Biomedical Materials Research. Part a·Shalu SuriAndrés J García
May 13, 2014·Chemical Communications : Chem Comm·Sufang MaBing Gong
Aug 25, 2015·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Artem A GorodetskyDenis A Komarov

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Citations

Mar 11, 2017·Chemical Communications : Chem Comm·Zhi-Heng LiLan He
Jul 7, 2020·Frontiers in Physiology·Emma GordonMaike Frye
Dec 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·A Loas, S J Lippard
Jun 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Qing WangXianshun Zeng
Jun 6, 2019·Biosensors·Shashi BhuckoryAllison M Dennis
Oct 8, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hao LiQin-Hua Song
Jan 10, 2018·Journal of the American Chemical Society·Christopher J ReinhardtJefferson Chan

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Methods Mentioned

BETA
FRET
Fluorescence
fluorescence imaging

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