Highly specific gene silencing by artificial microRNAs in Arabidopsis

The Plant Cell
Rebecca SchwabDetlef Weigel

Abstract

Plant microRNAs (miRNAs) affect only a small number of targets with high sequence complementarity, while animal miRNAs usually have hundreds of targets with limited complementarity. We used artificial miRNAs (amiRNAs) to determine whether the narrow action spectrum of natural plant miRNAs reflects only intrinsic properties of the plant miRNA machinery or whether it is also due to past selection against natural miRNAs with broader specificity. amiRNAs were designed to target individual genes or groups of endogenous genes. Like natural miRNAs, they had varying numbers of target mismatches. Previously determined parameters of target selection for natural miRNAs could accurately predict direct targets of amiRNAs. The specificity of amiRNAs, as deduced from genome-wide expression profiling, was as high as that of natural plant miRNAs, supporting the notion that extensive base pairing with targets is required for plant miRNA function. amiRNAs make an effective tool for specific gene silencing in plants, especially when several related, but not identical, target genes need to be downregulated. We demonstrate that amiRNAs are also active when expressed under tissue-specific or inducible promoters, with limited nonautonomous effects. Th...Continue Reading

Associated Datasets

References

May 29, 1992·Cell·D WeigelE M Meyerowitz
Sep 1, 1991·Molecular & General Genetics : MGG·M KoornneefJ H van der Veen
Aug 1, 1990·The Plant Cell·V F Irish, I M Sussex
Aug 1, 1990·The Plant Cell·D R SmythE M Meyerowitz
Mar 25, 1981·Journal of Molecular Biology·T F Smith, M S Waterman
Jul 27, 1999·Science·M A BuschD Weigel
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·A SessionsM F Yanofsky
Dec 3, 1999·Science·I KardailskyD Weigel
Apr 1, 2000·RNA·C FlammP Schuster
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C F Chuang, E M Meyerowitz
Apr 28, 2000·Nature·S J LiljegrenM F Yanofsky
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Dec 21, 2000·The Plant Journal : for Cell and Molecular Biology·R BornerS Melzer
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Jun 8, 2001·The Plant Journal : for Cell and Molecular Biology·K C ScortecciR M Amasino
Sep 29, 2001·The Plant Journal : for Cell and Molecular Biology·S V WesleyP M Waterhouse
Nov 28, 2001·The Plant Journal : for Cell and Molecular Biology·H A RoslanM X Caddick
May 4, 2002·Science·Philipp KapranovThomas R Gingeras
Jul 18, 2002·The Plant Cell·Cesar LlaveJames C Carrington
Aug 31, 2002·Cell·Matthew W RhoadesDavid P Bartel
Feb 8, 2003·Science·Robert M LarkinJoanne Chory
Feb 15, 2003·Developmental Cell·Kristin D KasschauJames C Carrington
May 20, 2003·Nature Biotechnology·Aimee L JacksonPeter S Linsley
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Aug 22, 2003·Nature·Javier F PalatnikDetlef Weigel
Sep 26, 2003·Nature·Yoontae LeeV Narry Kim
Oct 22, 2003·Cell·Dianne S SchwarzPhillip D Zamore
Oct 22, 2003·Cell·Anastasia KhvorovaSumedha D Jayasena
Oct 24, 2003·Development·Markus SchmidJan U Lohmann
Nov 1, 2003·Science·Kayoko YamadaJoseph R Ecker
Nov 15, 2003·Molecular Phylogenetics and Evolution·Annette Becker, Günter Theissen
Feb 6, 2004·Nature Biotechnology·Angela ReynoldsAnastasia Khvorova
Mar 12, 2004·Genes & Development·John G Doench, Phillip A Sharp

❮ Previous
Next ❯

Citations

Mar 15, 2011·Biotechnology Letters·Marlene ReichelAnthony A Millar
Mar 23, 2012·Biotechnology Letters·Xuming WangJianping Chen
Apr 10, 2013·Biotechnology Letters·Jie ZhouJianping Chen
Nov 16, 2011·Plant Molecular Biology·Cecilia Contreras-CubasJosé Luis Reyes
Dec 14, 2011·Plant Molecular Biology·Ying-Hsuan SunRonald R Sederoff
Jan 15, 2014·Plant Molecular Biology Reporter·Yan ChenGenji Qin
May 25, 2010·Molecular Biotechnology·Xuming WangJianping Chen
Apr 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Preeti Singh TeotiaNeeti Sanan Mishra
Nov 10, 2006·Nature Biotechnology·Juan Antonio García, Carmen Simón-Mateo
May 25, 2007·Nature Reviews. Molecular Cell Biology·G Venugopala ReddyElliot M Meyerowitz
Sep 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qianwen Sun, Dao-Xiu Zhou
Dec 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Taiowa A MontgomeryJames C Carrington
Apr 23, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hongqing GuoYanhai Yin
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cara H Haney, Sharon R Long
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·G ParryM Estelle
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ho-Ming ChenShu-Hsing Wu
Feb 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Miin-Feng WuDoris Wagner
Oct 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hiroyasu KitashibaMikhail E Nasrallah
Aug 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Samuel E WuestFrank Wellmer
Nov 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sandy VanderauweraFrank Van Breusegem
Mar 22, 2013·Molecular Biology of the Cell·Farid El KasmiGerd Jürgens
Jul 6, 2010·Bioinformatics·Mark E T Horn, Peter M Waterhouse
May 13, 2009·Journal of Experimental Botany·Sílvia CoimbraLuís Gustavo Pereira
Jan 19, 2010·Journal of Experimental Botany·Jiang WangZhenying Shi
Dec 22, 2010·Journal of Experimental Botany·G Le TrionnaireD Twell
Jan 24, 2012·Journal of Experimental Botany·Sébastien BesseauE Tapio Palva
Jan 28, 2012·Journal of Experimental Botany·Anna CzerednikGerco C Angenent

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.