Highly specific interactions between bHLH transcription factors and chromatin during retina development

Development
Dorota Skowronska-KrawczykJean-Marc Matter

Abstract

Basic helix-loop-helix (bHLH) transcription factors such as atonal homolog 5 (ATH5) and neurogenin 2 (NGN2) determine crucial events in retinogenesis. Using chromatin immunoprecipitation, we demonstrate that their interactions with target promoters undergo dynamic changes as development proceeds in the chick embryo. Chick ATH5 associates with its own promoter and with the promoter of the beta3 nicotinic receptor specifically in retinal ganglion cells and their precursors. NGN2 binds to the ATH5 promoter in retina but not in optic tectum, suggesting that interactions between bHLH factors and chromatin are highly tissue specific. The transcriptional activations of both promoters correlate with dimethylation of lysine 4 on histone H3. Inactivation of the ATH5 promoter in differentiated neurons is accompanied by replication-independent chromatin de-methylation. This report is one of the first demonstrations of correlation between gene expression, binding of transcription factors and chromatin modification in a developing neural tissue.

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Citations

Sep 1, 2011·Journal of Ocular Biology, Diseases, and Informatics·Rajesh C RaoShiming Chen
Jan 24, 2007·Molecular and Cellular Biology·Cindy Yen Okitsu, Chih-Lin Hsieh
Nov 2, 2005·Molecular and Cellular Biology·Dorota Skowronska-KrawczykJean-Marc Matter
Sep 9, 2009·Genome Biology·Marcel SourenJoachim Wittbrodt
Sep 26, 2007·PLoS Genetics·Filippo Del BeneJoachim Wittbrodt
Sep 15, 2015·Molecular Cell·Dorota Skowronska-KrawczykKang Zhang
Nov 8, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kathryn Leigh McCabeThomas A Reh
Feb 12, 2009·Genesis : the Journal of Genetics and Development·Amy N RiesenbergNadean L Brown
Aug 26, 2014·Mechanisms of Development·Rebecca SinnJoachim Wittbrodt
Sep 4, 2013·Neuroscience·G WilkinsonC Schuurmans
Dec 9, 2008·Developmental Biology·Minde I WillardsenMonica L Vetter
Mar 6, 2007·Developmental Biology·Ruben Adler, M Valeria Canto-Soler
Apr 3, 2007·Developmental Biology·Sophie Bel-VialarFabienne Pituello
May 29, 2016·Cellular and Molecular Life Sciences : CMLS·Sivadasan Bindu DhaneshJackson James
Apr 14, 2016·Development·Katharina LustJoachim Wittbrodt
Jun 23, 2018·Cell Cycle·Dorota Skowronska-Krawczyk
Apr 9, 2014·G3 : Genes - Genomes - Genetics·Chaquettea M Felton, Casonya M Johnson
Oct 9, 2019·International Journal of Molecular Sciences·Frédéric Blond, Thierry Léveillard
Nov 1, 2007·The Journal of Biological Chemistry·Julio HernandezJean-Marc Matter
Dec 6, 2018·Journal of Cellular Physiology·Rana ShafabakhshZatollah Asemi
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Joel B MiesfeldTom Glaser
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Mar 17, 2018·Progress in Retinal and Eye Research·Ximena Corso-DíazAnand Swaroop
Jan 29, 2005·Development·David A HutchesonMonica L Vetter
Aug 5, 2005·Development·Lidia Matter-SadzinskiJean-Marc Matter

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