Highly synergistic antimicrobial activity of magainin 2 and PGLa peptides is rooted in the formation of supramolecular complexes with lipids.

Scientific Reports
Christopher AisenbreyBurkhard Bechinger

Abstract

Magainin 2 and PGLa are cationic, amphipathic antimicrobial peptides which when added as equimolar mixture exhibit a pronounced synergism in both their antibacterial and pore-forming activities. Here we show for the first time that the peptides assemble into defined supramolecular structures along the membrane interface. The resulting mesophases are quantitatively described by state-of-the art fluorescence self-quenching and correlation spectroscopies. Notably, the synergistic behavior of magainin 2 and PGLa correlates with the formation of hetero-domains and an order-of-magnitude increased membrane affinity of both peptides. Enhanced membrane association of the peptide mixture is only observed in the presence of phophatidylethanolamines but not of phosphatidylcholines, lipids that dominate bacterial and eukaryotic membranes, respectively. Thereby the increased membrane-affinity of the peptide mixtures not only explains their synergistic antimicrobial activity, but at the same time provides a new concept to increase the therapeutic window of combinatorial drugs.

References

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M Zasloff
Sep 15, 1988·Biochimica Et Biophysica Acta·L Cruzeiro-Hansson, O G Mouritsen
Apr 1, 1987·The Biochemical Journal·M G GiovanniniD H Williams
Feb 18, 1972·Science·S J Singer, G L Nicolson
Jan 1, 1974·Journal of Supramolecular Structure·G Baumann, P Mueller
Jan 1, 1995·Annual Review of Microbiology·P Nicolas, A Mor
Oct 28, 1998·Biochemistry·K MatsuzakiK Miyajima
Mar 15, 2000·Journal of Molecular Biology·W P Russ, D M Engelman
Dec 20, 2002·Biopolymers·Yechiel Shai
May 28, 2003·Biochemistry·Katherine A Henzler WildmanA Ramamoorthy
Nov 3, 2004·Biochimica Et Biophysica Acta·Joachim Seelig
May 24, 2006·Biochimica Et Biophysica Acta·Ulrich H N DürrAyyalusamy Ramamoorthy
Aug 25, 2006·Biochimica Et Biophysica Acta·Burkhard Bechinger, Karl Lohner
Nov 21, 2007·Journal of Molecular Biology·Christopher AisenbreyGerhard Gröbner
Jul 15, 2009·Biochimica Et Biophysica Acta·Evgeniy SalnikovBurkhard Bechinger
Feb 16, 2010·The Journal of Chemical Physics·Paul W TumanengH L Scott
Mar 2, 2010·Biochimica Et Biophysica Acta·Radek Machán, Martin Hof
Jul 25, 2014·ACS Chemical Biology·Daniela RoversiLorenzo Stella
Aug 12, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Christopher Aisenbrey, Burkhard Bechinger
Dec 20, 2014·Journal of Peptide Science : an Official Publication of the European Peptide Society·Burkhard Bechinger
Jul 4, 2015·Current Topics in Medicinal Chemistry·Arnaud MarquetteBurkhard Bechinger
Dec 8, 2016·Biophysical Journal·Evgeniy S SalnikovBurkhard Bechinger
Jan 10, 2017·Journal of Peptide Science : an Official Publication of the European Peptide Society·Gilles MoulayBurkhard Bechinger
Oct 17, 2017·Scientific Reports·Jonathan ZerweckAnne S Ulrich
Apr 26, 2018·Biophysical Journal·Regina LeberGeorg Pabst
Aug 29, 2018·Methods in Molecular Biology·Sean McNamaraMarko E Horb
Sep 10, 2018·Biophysical Journal·Nicole Harmouche, Burkhard Bechinger

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Citations

Dec 19, 2020·International Journal of Molecular Sciences·Stanislav R KurpeOxana V Galzitskaya
Apr 13, 2021·Accounts of Chemical Research·Ivo Kabelka, Robert Vácha
Oct 28, 2020·The Journal of Physical Chemistry Letters·Jacob M RemingtonJianing Li
Sep 1, 2021·The Journal of Physical Chemistry. B·Aline CisseBurkhard Bechinger
Nov 5, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Laura Bedin DenardiJanio Morais Santurio

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Methods Mentioned

BETA
fluorescence correlation spectroscopy
FCS
FRET
NMR
cross-linking study
fluorescence

Software Mentioned

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