Highly thermostable endo-1,3-beta-glucanase (laminarinase) LamA from Thermotoga neapolitana: nucleotide sequence of the gene and characterization of the recombinant gene product.

Microbiology
Vladimir V ZverlovWolfgang H Schwarz

Abstract

The nucleotide sequence of clone pTT26 (3786 bp), containing the gene for 1,3-beta-glucanase LamA (laminarinase) from Thermotoga neapolitana, was determined. It contains an ORF encoding a protein of 646 aa (73328 Da). The central part of the protein is homologous to the complete catalytic domain of bacterial and some eukaryotic endo-1,3-beta-D-glucanases and belongs to family 16 of glycosyl hydrolases. This domain is flanked on both sides by one copy on each side of a substrate binding domain homologue (family II). The recombinant laminarinase protein was purified from Escherichia coli host cells in two forms, a 73 kDa and a processed 52 kDa protein, both having high specific activity towards laminarin (3100 and 2600 U mg-1, respectively) and K(m) values of 2.8 and 2.2 mg ml-1, respectively. Limited activity on 1,3-1,4-beta-glucan (lichenan) was detected (90 U mg-1). Laminarin was degraded in an endoglucanase modus, yielding glucose, laminaribiose and -triose as end products. Thus LamA classifies as an endo-1,3(4)-beta-glucanase (EC 3.2.1.6). The optimum temperature of the enzymes was 95 degrees C (73 kDa) and 85 degrees C (52 kDa) at an optimum pH of 6.2. The superior thermostability of the 73 kDa enzyme is demonstrated by inc...Continue Reading

References

Apr 1, 1975·FEBS Letters·M A Anderson, B A Stone
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Apr 1, 1995·Applied and Environmental Microbiology·K BronnenmeierW L Staudenbauer
Aug 16, 1993·Biochemical and Biophysical Research Communications·O N DakhovaG A Velikodvorskaya
Jan 1, 1995·Advances in Microbial Physiology·P TommeN R Gilkes
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·E S Bachman, D R McClay

❮ Previous
Next ❯

Citations

Sep 20, 2008·Applied Microbiology and Biotechnology·Yueh-Mei ChengMenghsiao Meng
Jan 11, 2001·Biochimica Et Biophysica Acta·A Planas
May 20, 1998·Current Opinion in Biotechnology·M W BauerR M Kelly
Jan 13, 2000·European Journal of Biochemistry·B O PetersenK K Thomsen
Dec 2, 2009·The Journal of Biological Chemistry·Amandine GasteboisThierry Fontaine
Oct 20, 2012·Protein Engineering, Design & Selection : PEDS·Wei-Chun LiuLie-Fen Shyur
Jul 23, 2008·Antimicrobial Agents and Chemotherapy·Maiken Cavling ArendrupCornelia Lass-Flörl
Feb 8, 2006·Applied and Environmental Microbiology·Dhaval M NanavatiKenneth M Noll
Jun 9, 2015·International Journal of Molecular Sciences·Nirakar PradhanAngelo Fontana
May 8, 2010·Fungal Genetics and Biology : FG & B·Thierry FontaineJean-Paul Latgé
Apr 28, 2009·Gene·Rustam M AbdeevEleonora S Piruzian
Apr 2, 2008·Annals of the New York Academy of Sciences·Amy L VanfossenRobert M Kelly
Aug 8, 2006·Biotechnology Journal·Assunta GiordanoAntonio Trincone
Mar 1, 2011·Biochemical and Biophysical Research Communications·Junio CotaFabio M Squina
Sep 6, 2002·Microbiology·Muriel BernardJean-Paul Latgé
Oct 27, 2006·FEMS Microbiology Reviews·Shannon B ConnersRobert M Kelly
Oct 15, 2013·FEMS Microbiology Reviews·Sara E Blumer-SchuetteRobert M Kelly
Jul 14, 2010·Fungal Genetics and Biology : FG & B·Lukas HartlJean-Paul Latgé
Feb 11, 2015·Acta Crystallographica. Section F, Structural Biology Communications·Yury A KislitsynGalina A Velikodvorskaya
Jun 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiulan WuXiangru Liao
Feb 15, 2000·Molecular Microbiology·S Shahinian, H Bussey
Mar 15, 2014·The Journal of Biological Chemistry·Thomas StalhbergerThierry Fontaine
Sep 13, 2020·Applied and Environmental Microbiology·Jian YangMasaru Tanokura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.