Hijacking of multiple phospholipid biosynthetic pathways and induction of membrane biogenesis by a picornaviral 3CD protein

PLoS Pathogens
Sravani BanerjeeCraig E Cameron

Abstract

RNA viruses induce specialized membranous structures for use in genome replication. These structures are often referred to as replication organelles (ROs). ROs exhibit distinct lipid composition relative to other cellular membranes. In many picornaviruses, phosphatidylinositol-4-phosphate (PI4P) is a marker of the RO. Studies to date indicate that the viral 3A protein hijacks a PI4 kinase to induce PI4P by a mechanism unrelated to the cellular pathway, which requires Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1, GBF1, and ADP ribosylation factor 1, Arf1. Here we show that a picornaviral 3CD protein is sufficient to induce synthesis of not only PI4P but also phosphatidylinositol-4,5-bisphosphate (PIP2) and phosphatidylcholine (PC). Synthesis of PI4P requires GBF1 and Arf1. We identified 3CD derivatives: 3CDm and 3CmD, that we used to show that distinct domains of 3CD function upstream of GBF1 and downstream of Arf1 activation. These same 3CD derivatives still supported induction of PIP2 and PC, suggesting that pathways and corresponding mechanisms used to induce these phospholipids are distinct. Phospholipid induction by 3CD is localized to the perinuclear region of the cell, the outcome of which is...Continue Reading

References

Oct 1, 1996·Journal of Virology·A SchlegelK Kirkegaard
Jul 15, 2003·Journal of Virology·Paul AhlquistBilly T Dye
Jan 7, 2005·The Journal of Biological Chemistry·Kelly M WeixelOra A Weisz
May 12, 2005·PLoS Biology·William T JacksonKarla Kirkegaard
Jun 28, 2005·Biochimica Et Biophysica Acta·Julie G DonaldsonRoberto Weigert
Dec 17, 2005·Analytical Biochemistry·Jamie J ArnoldMichael R Mattern
Jul 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jamil S SaadMichael F Summers
Sep 29, 2006·Journal of Virology·Els WesselsFrank J M van Kuppeveld
Jun 15, 2007·Journal of Virology·George A BelovEllie Ehrenfeld
Sep 7, 2007·Journal of Virology·Matthew P Taylor, Karla Kirkegaard
Dec 21, 2007·Autophagy·Matthew P Taylor, Karla Kirkegaard
Feb 3, 2009·Nature Chemical Biology·José B SáenzDavid B Haslam
Jun 11, 2009·The Biochemical Journal·Gerald R V HammondRobin F Irvine
Sep 3, 2009·Future Microbiology·Joyce JoseRichard J Kuhn
Nov 6, 2009·Journal of Cell Science·Iman van den Bout, Nullin Divecha
Jun 5, 2010·Future Microbiology·Craig E CameronIbrahim M Moustafa
Jul 20, 2010·Cell Host & Microbe·Johan A den BoonPaul Ahlquist
Sep 10, 2010·Annual Review of Microbiology·Johan A den Boon, Paul Ahlquist
Oct 14, 2011·Viruses·Kathryn A Klein, William T Jackson
Nov 11, 2011·Journal of Virology·George A BelovEllie Ehrenfeld
Nov 30, 2011·The EMBO Journal·Jun SasakiKoki Taniguchi
Jan 20, 2012·Journal of Virology·Alexander L GreningerJoseph L Derisi
May 29, 2012·Trends in Biochemical Sciences·Nihal Altan-Bonnet, Tamas Balla
Jun 14, 2013·Journal of Virology·Alexsia L Richards, William T Jackson
Aug 1, 2013·Physiological Reviews·Tamas Balla
Aug 8, 2013·Critical Reviews in Microbiology·Jing LuMing-Liang He
Dec 20, 2013·Journal of Virology·Cristina M DorobantuFrank J M van Kuppeveld

❮ Previous
Next ❯

Citations

Jan 7, 2020·The American Journal of Chinese Medicine·Meng-Jie WangYuan-Yuan Liu
Mar 20, 2020·Microbiology and Molecular Biology Reviews : MMBR·Steven M PascalKomala Ponniah
Sep 26, 2019·Frontiers in Microbiology·Di SunXiaoyue Chen
Mar 14, 2019·Frontiers in Microbiology·Zhenlu ZhangXiaofeng Wang
Oct 1, 2019·Annual Review of Virology·Ellen Ketter, Glenn Randall
Jun 20, 2019·Journal of Biomedical Science·Collins Oduor Owino, Justin Jang Hann Chu
Sep 26, 2020·Frontiers in Microbiology·Xinhong LiXiaoyue Chen
Jan 28, 2021·Viruses·Christine E Peters, Jan E Carette
Apr 4, 2021·Viruses·Terry Jackson, Graham J Belsham
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Louise H WongEmily R Eden

❮ Previous
Next ❯

Methods Mentioned

BETA
transfection
pull-down
transmission electron microscopy
transmission microscopy
GTPases
GTPase
PCR
electrophoresis
transfections
protein assay

Software Mentioned

Zeiss
AxioVision LE64
GraphPad Prism
ZEN

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.