HILIC-MRM-MS for Linkage-Specific Separation of Sialylated Glycopeptides to Quantify Prostate-Specific Antigen Proteoforms.

Journal of Proteome Research
Yuri E M van der BurgtL Renee Ruhaak

Abstract

Elevated serum prostate-specific antigen (PSA) levels in body fluids may indicate prostate cancer (PCa), but it is noted that the clinical performance is rather poor. Specificity and sensitivity values of 20 and 94% at a cutoff value of 4.1 ng/mL, respectively, result in overdiagnosis and unnecessary interventions. Previous exploratory studies have indicated that the glycosylation of PSA potentially leads to improved PCa diagnosis based on qualitative analyses. However, the applied methods are not suited for a quantitative evaluation or implementation in a medical laboratory. Therefore, in this proof-of-principle study, we have evaluated the use of hydrophilic interaction liquid chromatography (HILIC) in combination with targeted quantitative mass spectrometry for the sialic acid linkage-specific analysis of PSA glyco-proteoforms based on either trypsin or ArgC peptides. The efficiency of PSA proteolysis was optimized as well as the glycopeptide separation conditions (buffer type, strength, and pH). The HILIC-based analysis of PSA glyco-proteoforms presented here has the potential for the clinical validation of patient cohorts. The method shows the feasibility of the use of a HILIC stationary phase for the separation of isomeri...Continue Reading

References

Jul 7, 2005·JAMA : the Journal of the American Medical Association·Ian M ThompsonCharles A Coltman
Nov 21, 2007·Cancer Causes & Control : CCC·Ruth EtzioniEric Feuer
Feb 26, 2008·Journal of Chromatography. a·Tohru IkegamiNobuo Tanaka
Oct 21, 2011·Nature
Jan 22, 2013·Journal of Chromatography. a·Abhinav KumarDavid V McCalley
Oct 1, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Andrea R HorvathUNKNOWN Test Evaluation Working Group of the European Federation of Clinical Chemistry Laboratory Medicine
Dec 3, 2013·Journal of Biomolecular Techniques : JBT·Darryl JohnsonRon Orlando
Feb 8, 2014·Analytical Chemistry·Qiuting HongCarlito B Lebrilla
May 13, 2014·Biochemical and Biophysical Research Communications·Tohru YoneyamaYuki Tobisawa
Dec 17, 2014·Journal of Proteome Research·Irene van den BroekChrista M Cobbaert
Feb 27, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Pauline BrosChristophe Hirtz
Mar 3, 2015·Advances in Cancer Research·Richard R DrakeJulius O Nyalwidhe
Apr 29, 2016·Nature Protocols·Piero GiansantiAlbert J R Heck
Jul 5, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·Phillip J MonaghanUNKNOWN Test Evaluation Working Group of the European Federation of Clinical Chemistry and Laboratory Medicine
Sep 2, 2016·Journal of Biomolecular Techniques : JBT·Yining HuangRon Orlando
Dec 3, 2016·Analytical and Bioanalytical Chemistry·Kathirvel AlagesanDaniel Kolarich
Apr 20, 2017·International Journal of Molecular Sciences·Montserrat Ferrer-BatalléRosa Peracaula
Jun 13, 2017·Analytical Chemistry·Christopher M ShufordRussell P Grant
Feb 6, 2018·Journal of Chromatography. a·Andrew J Alpert
Mar 6, 2018·Analytical Chemistry·Guinevere S M KammeijerManfred Wuhrer
Mar 20, 2018·Chemical Reviews·L Renee RuhaakCarlito B Lebrilla
Apr 24, 2018·Glycoconjugate Journal·Zejian ZhangStephanie Holst
May 1, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·L Renee RuhaakChrista M Cobbaert
May 29, 2018·Analytical and Bioanalytical Chemistry·Petr KozlikMiloslav Sanda
Sep 7, 2018·BMJ : British Medical Journal·Kari A O TikkinenThomas Agoritsas
Oct 3, 2018·Clinical Chemistry·Andrew J Vickers
Nov 30, 2018·Advances in Experimental Medicine and Biology·Kathirvel AlagesanDaniel Kolarich
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Oct 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katarina Molnarova, Petr Kozlík
Dec 9, 2020·Journal of Pharmaceutical and Biomedical Analysis·Balazs ReiderAndras Guttman
Jan 14, 2021·Biochemical Society Transactions·Anastasia ChernykhMorten Thaysen-Andersen
Feb 11, 2021·Mass Spectrometry Reviews·Milani W PatabandigeHeather Desaire
Jun 25, 2020·Molecular & Cellular Proteomics : MCP·Daniel G Delafield, Lingjun Li
Jun 3, 2021·International Journal of Molecular Sciences·Caterina GabrieleMarco Gaspari

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.