Hindering the formation and promoting the dispersion of medical biofilms: non-lethal effects of seagrass extracts

BMC Complementary and Alternative Medicine
Luca De VincentiJutta Papenbrock

Abstract

Biofilms have great significance in healthcare-associated infections owing to their inherent tolerance and resistance to antimicrobial therapies. New approaches to prevent and treat unwanted biofilms are urgently required. To this end, three seagrass species (Enhalus acoroides, Halophila ovalis and Halodule pinifolia) collected in Vietnam and in India were investigated for their effects in mediating non-lethal interactions on sessile bacterial (Escherichia coli) and fungal (Candida albicans) cultures. The present study was focused on anti-biofilm activities of seagrass extracts, without killing cells. Methanolic extracts were characterized, and major compounds were identified by MS/MS analysis. The antibiofilm properties of the seagrass extracts were tested at sub-lethal concentrations by using microtiter plate adhesion assay. The performance of the most promising extract was further investigated in elegant bioreactors to reproduce mature biofilms both at the solid/liquid and the solid/air interfaces. Dispersion and bioluminescent assays were carried out to decipher the mode of action of the bioactive extract. It was shown that up to 100 ppm of crude extracts did not adversely affect microbial growth, nor do they act as a carbo...Continue Reading

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Citations

Jul 9, 2020·Microorganisms·Kyle S LandrySandip S Panesar
Jun 4, 2019·Journal of Environmental Management·Cristina CattòMarco Saracchi
Jun 3, 2021·Pharmaceutics·Giuliana DonadioFabrizio Dal Piaz

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Datasets Mentioned

BETA
EA20130301

Methods Mentioned

BETA
environmental stress

Software Mentioned

MassBank
MATLAB
ReSpect
GraphPad Prism

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