Hinge-Region O-Glycosylation of Human Immunoglobulin G3 (IgG3).

Molecular & Cellular Proteomics : MCP
Rosina PlompM Wuhrer

Abstract

Immunoglobulin G (IgG) is one of the most abundant proteins present in human serum and a fundamental component of the immune system. IgG3 represents ∼8% of the total amount of IgG in human serum and stands out from the other IgG subclasses because of its elongated hinge region and enhanced effector functions. This study reports partial O-glycosylation of the IgG3 hinge region, observed with nanoLC-ESI-IT-MS(/MS) analysis after proteolytic digestion. The repeat regions within the IgG3 hinge were found to be in part O-glycosylated at the threonine in the triple repeat motif. Non-, mono- and disialylated core 1-type O-glycans were detected in various IgG3 samples, both poly- and monoclonal. NanoLC-ESI-IT-MS/MS with electron transfer dissociation fragmentation and CE-MS/MS with CID fragmentation were used to determine the site of IgG3 O-glycosylation. The O-glycosylation site was further confirmed by the recombinant production of mutant IgG3 in which potential O-glycosylation sites had been knocked out. For IgG3 samples from six donors we found similar O-glycan structures and site occupancies, whereas for the same samples the conserved N-glycosylation of the Fc CH2 domain showed considerable interindividual variation. The occupancy...Continue Reading

References

Jan 1, 1975·Scandinavian Journal of Immunology·P M JohnsonP M Scopes
Apr 15, 1991·The Biochemical Journal·I B WilsonG von Heijne
May 1, 1990·Infection and Immunity·J ReinholdtM Kilian
Jul 1, 1969·The Journal of Allergy·S C MinesP Fireman
May 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·G M EdelmanM J Waxdal
Apr 1, 1970·The Journal of Clinical Investigation·A MorellT A Waldmann
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·N TakahashiF W Putnam
Apr 14, 1982·Biochemical and Biophysical Research Communications·T TakayasuE Munekata
Jan 10, 2002·European Journal of Human Genetics : EJHG·P DardA Sanchez-Mazas
Aug 5, 2006·Science·Yoshikatsu KanekoJeffrey V Ravetch
Nov 9, 2007·Biochimica Et Biophysica Acta·Mads Agervig Tarp, Henrik Clausen
Oct 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Randall J BrezskiRobert E Jordan
Jan 16, 2010·MAbs·Roy Jefferis, Marie-Paule Lefranc
Dec 15, 2010·Current Opinion in Organ Transplantation·Robert M Anthony, Falk Nimmerjahn
Mar 31, 2012·Nature Reviews. Drug Discovery·P Mark Hogarth, Geoffrey A Pietersz
Jul 21, 2012·Journal of Biotechnology·Amelie CrosetBruno Antonsson
Jan 10, 2013·Journal of Proteome Research·Maja Pučić BakovićManfred Wuhrer
Jul 23, 2013·Methods : a Companion to Methods in Enzymology·Tom VinkRob N de Jong
Jul 19, 2014·Journal of Molecular Biology·Alysia A AhmedPamela J Bjorkman
Nov 5, 2014·Frontiers in Immunology·Gestur VidarssonTheo Rispens

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Citations

Oct 27, 2016·Electrophoresis·Anna TýčováKarel Klepárník
Nov 23, 2016·Scientific Reports·Gillian DekkersGestur Vidarsson
Mar 25, 2016·Molecular & Cellular Proteomics : MCP·Rosina PlompManfred Wuhrer
Nov 26, 2015·Molecular & Cellular Proteomics : MCP·Marcus HoffmannErdmann Rapp
Nov 23, 2017·Molecular & Cellular Proteomics : MCP·Zsuzsanna Darula, Katalin F Medzihradszky
Oct 25, 2017·Nature Reviews. Immunology·Lenette L LuGalit Alter
Mar 6, 2019·Protein Science : a Publication of the Protein Society·Seiji SaitoShigeru Iida
Jul 28, 2019·MAbs·Kathrin StavenhagenManfred Wuhrer
Jan 16, 2020·Applied Microbiology and Biotechnology·Ziyan WangHuili Lu
Jul 10, 2019·Glycobiology·Noortje de HaanManfred Wuhrer
Oct 17, 2019·Glycobiology·Jonathan SjögrenAndreas Nägeli
Aug 23, 2019·Journal of Nanobiotechnology·Oleksii O PeltekAlexander S Timin
Nov 12, 2015·Glycoconjugate Journal·Florent ClercManfred Wuhrer
Dec 8, 2015·Glycoconjugate Journal·Lucia F Zacchi, Benjamin L Schulz
Feb 7, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fleur S van de BovenkampYoann Rombouts
Aug 16, 2017·Scientific Reports·Myrthe E SonneveldGestur Vidarsson
Nov 20, 2018·Frontiers in Immunology·Matthew J BuettnerKevin J Yarema
Sep 30, 2016·Biochemistry. Biokhimii︠a︡·Y L DorokhovT V Komarova
Aug 21, 2016·Analytical Methods : Advancing Methods and Applications·Jude C LakbubHeather Desaire
Dec 16, 2019·Immunogenetics·Diego Garzón-Ospina, Sindy P Buitrago
Aug 15, 2020·Frontiers in Bioengineering and Biotechnology·Somanath KallolimathHerta Steinkellner
Feb 20, 2021·MAbs·Thach H ChuMargaret E Ackerman
Feb 13, 2019·Trends in Immunology·Timon DamelangAmy W Chung
Apr 27, 2021·Frontiers in Pharmacology·Riccardo CapecchiLorenzo Bertani
Dec 22, 2017·Molecular Immunology·Gillian DekkersGestur Vidarsson
Jul 25, 2021·The Journal of Biological Chemistry·Valentina A SpiteriStephen J Perkins
Aug 20, 2020·Journal of the American Society for Mass Spectrometry·Kristina SrzentićMowei Zhou
Feb 25, 2020·Analytical Chemistry·Ana MomčilovićViktoria Dotz
Oct 6, 2021·Clinical Journal of the American Society of Nephrology : CJASN·Xue ZhangJing Jin

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Methods Mentioned

BETA
glycosylation
gel filtration
ELISA
electrophoresis
acetylation

Software Mentioned

MASCOT Daemon
IDCalc
Compounds
Protein Prospector MS - Product
ExPASY FindPept
MASCOT
DataAnalysis
- AutoMS

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