Hinokitiol up-regulates miR-494-3p to suppress BMI1 expression and inhibits self-renewal of breast cancer stem/progenitor cells

Oncotarget
Shih-Ming ChenWen-Wei Chang

Abstract

Hinokitiol (β-thujaplicin) is a tropolone-related compound that has anti-microbe, anti-inflammation, and anti-tumor effects. Cancer stem/progenitor cells (CSCs) are a subpopulation of cancer cells with tumor initiation, chemoresistant, and metastatic properties and have been considered the important therapeutic target in future cancer therapy. Previous studies reported that hinokitiol exhibits an anti-cancer activity against murine tumor cells through the induction of autophagy. The current research revealed that hinokitiol suppressed the self-renewal capabilities of human breast CSCs (BCSCs) and inhibited the expression of BMI1 at protein level without suppressing its mRNA. Treatment of hinokitiol in mammospheres induced the expression of miR-494-3p and inhibition of miR-494-3p expression in BCSCs. This treatment abolished the suppressive effects of hinokitiol in mammosphere formation and BMI1 expression. BMI1 is a target of miR-494-3p by luciferase-based 3'UTR reporter assay. Overexpression of miR-494-3p in BCSCs caused the down-regulation of BMI1 protein, inhibition of mammosphere forming capability, and suppression of their tumorigenicity. Moreover, miR-494-3p expression was significantly and inversely correlated with patie...Continue Reading

References

Nov 2, 2001·Nature·T ReyaI L Weissman
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
May 21, 2003·Genes & Development·Gabriela DontuMax S Wicha
Sep 28, 2006·Annual Review of Medicine·Piero DalerbaMichael F Clarke
Oct 12, 2007·Biocontrol Science·Yasuhiro MoritaNakao Ishida
Feb 27, 2009·Journal of the National Cancer Institute·Erina VlashiFrank Pajonk
Apr 8, 2010·Seminars in Cancer Biology·Patrick C HermannChristopher Heeschen
Nov 2, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Won Kyu KimHoguen Kim
Mar 2, 2013·Molecular Cancer Research : MCR·William Weidong DuAlbert J Yee
Aug 6, 2013·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lionel LimAndrei Goga
Jun 4, 2014·British Journal of Cancer·G LinY-L Chung
Nov 5, 2014·Future Medicinal Chemistry·Claire BouvardShoutian Zhu
Jan 28, 2015·Neoplasia : an International Journal for Oncology Research·Louis-Bastien WeiswaldVirginie Dangles-Marie
Apr 4, 2015·Seminars in Oncology·Jaffer A AjaniIra B Steinberg
May 16, 2015·Clinical Epigenetics·Pedro Costa-PinheiroCarmen Jerónimo
Jan 29, 2016·International Journal of Oncology·Vishwa Jeet AmatyaYukio Takeshima
Jul 12, 2016·International Journal of Molecular Sciences·Jui-Hung WengYu-Liang Kuo
Dec 17, 2016·Oncotarget·Alice FaversaniSilvano Bosari
Nov 9, 2017·Molecular Medicine Reports·Zhiwei LiaoJian Dong

❮ Previous
Next ❯

Citations

Jul 19, 2018·Biomedicines·Judy S Crabtree, Lucio Miele
Dec 12, 2018·Journal of Cellular Physiology·Zahra AsadzadehBehzad Baradaran
Feb 8, 2020·Cancers·Pei-Ling HsiehCheng-Chia Yu
Aug 4, 2018·Journal of Applied Toxicology : JAT·Meran Keshawa EdiriweeraSameera Ranganath Samarakoon
Dec 18, 2018·Natural Product Reports·Huijuan GuoChristine Beemelmanns
Sep 11, 2019·Toxicological Sciences : an Official Journal of the Society of Toxicology·Zhishan WangChengfeng Yang
Jan 9, 2021·Cancer Cell International·Tong NiuWei Xiao

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE37405

Methods Mentioned

BETA
xenograft
transfection
FACS
protein assay
PCR
flow cytometry

Software Mentioned

PROGmiR
MICRORNA
ImageJ
MIRUMIR
Smartflare
GraFit

Related Concepts

Related Feeds

Autophagy & Disease

Autophagy is an important cellular process for normal physiology and both elevated and decreased levels of autophagy are associated with disease. Here is the latest research.

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Breast Cancer: Therapeutic Approaches

Several different therapeutic approaches are used to treat breast cancer. These include chemotherapy, hormonal therapy, targeted therapy, and Immunotherapy. Discover the latest research on breast cancer therapeutic approaches here.