Hint1 expression inhibits proliferation and promotes radiosensitivity of human SGC7901 gastric cancer cells

Oncology Letters
Xiaowei WeiJinfei Chen

Abstract

Gastric cancer is a prevalent, malignant tumor that frequently escapes treatment. Histidine triad nucleotide-binding protein 1 (Hint1) is a haploinsufficient tumor suppressor gene which contributes to intercellular communication, helps to regulate cell proliferation and survival, and is frequently underexpressed in gastric cancer. To examine the involvement of Hint1 in gastric cancer, small interfering RNA was used to knock down Hint1 expression in the human gastric cancer cell line SGC-7901. The data revealed that Hint1 inhibited cell proliferation, reduced radiation-induced DNA damage repair and caused G1 phase arrest, which increased the radiosensitivity of gastric cancer cells. Further mechanistic studies revealed a novel function of Hint1, whereby it acted as a negative regulator of extracellular signal-regulated kinase. These results demonstrated the critical function of Hint1 in the biology of human gastric cancer. Acting as a tumor growth suppressor and a radiosensitive agent, this protein is a potential biomarker and may be an attractive target for specific therapeutic interventions against gastric cancer.

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tao SuI Bernard Weinstein
Sep 28, 2005·Oncogene·H LiI B Weinstein
Jul 13, 2006·The Journal of Biological Chemistry·Jörg Weiske, Otmar Huber
May 19, 2007·Cancer Research·Lin WangI Bernard Weinstein
Oct 15, 2008·The Journal of Cell Biology·Haiyang LiI Bernard Weinstein
Nov 3, 2010·Advances in Cancer Research·Joanne SmithDavid A Gillespie
Mar 18, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yasunari MizumotoMasaki Inoue
Apr 7, 2011·International Journal of Oncology·Huaying HuangJinfei Chen
Jul 8, 2011·Nature Reviews. Cancer·Elizabeth A MusgroveRobert L Sutherland
Oct 20, 2012·Cancer Cell·Yoon Jong ChoiPiotr Sicinski
Jan 23, 2013·Breast Cancer Research : BCR·Emmi PeuralaArja Jukkola-Vuorinen
Aug 27, 2013·Cell Cycle·Tsutomu ShimuraNaoki Kunugita
Dec 2, 2014·Nature Reviews. Drug Discovery·Ahmed A Samatar, Poulikos I Poulikakos

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Citations

Sep 5, 2020·OncoTargets and Therapy·Dong-Dong DuanMeng-Qiao Zhou
Mar 7, 2021·Diagnostics·Carolina Carrillo-NajarAlma Ortiz-Plata
Dec 1, 2021·Journal of Proteome Research·Allen S HoJennifer E Van Eyk

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Methods Mentioned

BETA
X-ray
Assay
protein assay
PCR
fluorescence-activated cell sorting

Software Mentioned

SPSS
ModFit LT
CaspLab

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