Hippocampal "cholinergic interneurons" visualized with the choline acetyltransferase promoter: anatomical distribution, intrinsic membrane properties, neurochemical characteristics, and capacity for cholinergic modulation

Frontiers in Synaptic Neuroscience
Feng YiJosh Lawrence

Abstract

Release of acetylcholine (ACh) in the hippocampus (HC) occurs during exploration, arousal, and learning. Although the medial septum-diagonal band of Broca (MS-DBB) is the major extrinsic source of cholinergic input to the HC, cholinergic neurons intrinsic to the HC also exist but remain poorly understood. Here, ChAT-tauGFP and ChAT-CRE/Rosa26YFP (ChAT-Rosa) mice were examined in HC. The HC of ChAT-tauGFP mice was densely innervated with GFP-positive axons, often accompanied by large GFP-positive structures, some of which were Neurotrace/DAPI-negative and likely represent large axon terminals. In the HC of ChAT-Rosa mice, ChAT-YFP cells were Neurotrace-positive and more abundant in CA3 and dentate gyrus than CA1 with partial overlap with calretinin/VIP. Moreover, an anti-ChAT antibody consistently showed ChAT immunoreactivity in ChAT-YFP cells from MS-DBB but rarely from HC. Furthermore, ChAT-YFP cells from CA1 stratum radiatum/stratum lacunosum moleculare (SR/SLM) exhibited a stuttering firing phenotype but a delayed firing phenotype in stratum pyramidale (SP) of CA3. Input resistance and capacitance were also different between CA1 SR/LM and CA3 SP ChAT-YFP cells. Bath application of ACh increased firing frequency in all ChAT-Y...Continue Reading

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Citations

Jul 30, 2015·Neuropharmacology·Adam J GrangerBernardo L Sabatini
Mar 26, 2016·Frontiers in Synaptic Neuroscience·Jan Krzysztof Blusztajn, Jasmine Rinnofner
Jul 28, 2015·Biochemical Pharmacology·Qing Cheng, Jerrel L Yakel
Mar 29, 2019·Scientific Reports·Antoine D MadarMathew V Jones
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Nov 14, 2015·Brain Structure & Function·Robert GábrielMárta Wilhelm
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Oct 7, 2015·Frontiers in Synaptic Neuroscience·Sukumar Vijayaraghavan, Geeta Sharma
Jun 27, 2019·Progress in Retinal and Eye Research·Muneeb A FaiqKevin C Chan

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Methods Mentioned

BETA
transgenic

Software Mentioned

Excel
Image J
Neurotrace
Photoshop
Prism
ImageJ
Fluoview

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