Hippocampal neuropeptide Y protein expression following controlled cortical impact and posttraumatic epilepsy

Epilepsy & Behavior : E&B
Zhuopin SunKevin M Kelly

Abstract

This study assessed neuropeptide Y (NPY) expression in the hippocampus after long-term survival following traumatic brain injury (TBI) induced by controlled cortical impact (CCI) with or without the development of posttraumatic epilepsy (PTE). We hypothesized that following long-term survival after CCI, the severity of tissue injury and the development of PTE would correlate with the degree of hippocampal neurodegeneration as reflected by NPY+ and neuronal nuclear antigen (NeuN)+ cell loss. Adult Sprague-Dawley rats of 2-3 months of age were lesioned in the right parietal cortex and monitored for seizure activity by video and/or video-EEG. Neuropeptide Y and NeuN immunoreactivities (IRs) were quantified by light microscopy and semiautomatic image analysis approaches for unbiased quantification. Severely injured animals, marked by extensive tissue loss in the ipsilateral neocortex and adjacent hippocampus, resulted in significantly lower NeuN+ hilar cell density and NPY+ cell loss in the contralateral Cornu Ammonis (CA)-3 and dentate hilus (DH). The degree of NPY+ cell loss was more severe in CCI-injured animals with PTE than those animals that did not develop PTE. Mildly injured animals demonstrated no significant change of NPY...Continue Reading

Citations

Apr 3, 2019·Experimental and Therapeutic Medicine·Zheng GuYaqiong Han
Aug 3, 2020·European Journal of Trauma and Emergency Surgery : Official Publication of the European Trauma Society·José Luís AlvesAnabela Mota Pinto
Nov 27, 2020·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Nuan WangXingshun Xu

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