hiPSC-derived hepatocytes closely mimic the lipid profile of primary hepatocytes: A future personalised cell model for studying the lipid metabolism of the liver

Journal of Cellular Physiology
Mostafa KiamehrKatriina Aalto-Setälä

Abstract

Hepatocyte-like cells (HLCs) differentiated from human-induced pluripotent stem cells offer an alternative platform to primary human hepatocytes (PHHs) for studying the lipid metabolism of the liver. However, despite their great potential, the lipid profile of HLCs has not yet been characterized. Here, we comprehensively studied the lipid profile and fatty acid (FA) metabolism of HLCs and compared them with the current standard hepatocyte models: HepG2 cells and PHHs. We differentiated HLCs by five commonly used methods from three cell lines and thoroughly characterized them by gene and protein expression. HLCs generated by each method were assessed for their functionality and the ability to synthesize, elongate, and desaturate FAs. In addition, lipid and FA profiles of HLCs were investigated by both mass spectrometry and gas chromatography and then compared with the profiles of PHHs and HepG2 cells. HLCs resembled PHHs by expressing hepatic markers: secreting albumin, lipoprotein particles, and urea, and demonstrating similarities in their lipid and FA profile. Unlike HepG2 cells, HLCs contained low levels of lysophospholipids similar to the content of PHHs. Furthermore, HLCs were able to efficiently use the exogenous FAs avai...Continue Reading

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Citations

Jul 16, 2020·Tissue Engineering and Regenerative Medicine·Sara DarakhshanAbbas Piryaei
Dec 6, 2019·Frontiers in Medicine·James L Corbett, Stephen A Duncan
Aug 10, 2020·Scientific Reports·Stefano ManziniGiulia Chiesa
Aug 4, 2019·International Journal of Molecular Sciences·Mostafa KiamehrKati Juuti-Uusitalo
Nov 21, 2020·PLoS Neglected Tropical Diseases·Kessiri KongmanasPanisadee Avirutnan

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