Abstract
Human blood leukocytes were challenged by IgE-dependent stimuli (antigens or anti-IgE antibodies) and the chemotactic agonists C5a, N-formyl-Met-Leu-Phe (fMLP) or NAF, a novel neutrophil-activating peptide produced by stimulated human monocytes. IgE-dependent stimuli induced abundant release of histamine and sulfidoleukotrienes (LTC, LTD and LTE). The effects of the chemotactic peptides differed considerably: C5a induced high percentages of histamine release already at concentrations as low as 10(-9) M, but no leukotriene formation: NAF was completely inactive up to 10(-6)M, and fMLP induced moderate release of both products at relatively high concentration only (1-2.5 x 10(-6) M). All three chemotactic peptides, however, induced a concentration-dependent release of elastase from cytochalasin-B-treated human neutrophils. The EC50 was approximately 0.3, 2 and 5 x 10(-9) M for C5a, NAF and fMLP, respectively. NAF showed activity over a wider concentration range and its dose-response curve was less steep than that of the two other agonists. Our results suggest that NAF, that may be generated at the sites of hypersensitivity reactions, is likely to induced the immigration of neutrophils, but not the direct activation of basophils a...Continue Reading
Citations
Nov 18, 2000·British Journal of Pharmacology·T C TheoharidesA Athanasiou
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S C BischoffC A Dahinden
Apr 4, 2000·American Journal of Respiratory Cell and Molecular Biology·M M ChangR Wu
Jun 13, 1994·FEBS Letters·M U EhrengruberD A Deranleau
Sep 29, 2011·Cellular Immunology·Shuichiro EndoEdward G Brooks
Nov 28, 2007·International Immunopharmacology·Richard G DiScipio, Ingrid U Schraufstatter
Sep 3, 2013·Allergy·U BlankG Nilsson
Apr 1, 1992·European Journal of Immunology·S TakafujiC A Dahinden
Nov 1, 1991·The Journal of Allergy and Clinical Immunology·J A GrantM A Lett-Brown
Feb 13, 2008·International Archives of Allergy and Immunology·A L de WeckJ Sainte-Laudy
Nov 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dorothea Dijkstra, Almut Meyer-Bahlburg
Dec 1, 1996·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J DouglassS T Holgate
Jul 10, 2004·Blood·David E SloaneJonathan P Arm
Aug 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W J LieA T J Tool
Feb 1, 1991·European Journal of Immunology·Y KurimotoC A Dahinden