Histamine downregulates aquaporin 5 in human nasal epithelial cells

American Journal of Rhinology & Allergy
Weiwei WangRuitao Zhang

Abstract

Aquaporin 5 (AQP5) is a water-specific channel protein. It is thought to be a key participant in fluid secretion and a rate-limiting barrier to the secretion seen during allergic inflammation. We sought to determine the effect of histamine on AQP5 expression in human nasal epithelial cells (HNEpC). HNEpC cells were cultured with four concentrations of histamine in vitro. The phosphorylation of cyclic adenosine monophosphate-responsive element binding protein (CREB) at serine 133 and the AQP5 protein were measured by using immunocytochemistry and Western blotting. Real-time polymerase chain reaction was used to detect AQP5 messenger ribonucleic acid (mRNA). Concentration-dependent histamine induced-inhibition of CREB phosphorylation at serine 133 in HNEpC cells was observed, and AQP5 mRNA and protein were also downregulated in a concentration-dependent fashion. Histamine downregulates AQP5 production in HNEpC cells by inhibiting CREB phosphorylation at serine 133.

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Citations

May 16, 2015·American Journal of Rhinology & Allergy·Matthew A Rank
Jan 5, 2019·Clinical Science·Alessandro PiniArianna Carolina Rosa
Aug 23, 2020·International Journal of Molecular Sciences·Su Young JungSeung Geun Yeo

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