Histamine H-1 binding site on human polymorphonuclear leukocytes

Inflammation
S Wescott, M Kaliner

Abstract

Neutrophilic polymorphonuclear leukocytes (PMN) infiltrate the sites of allergic reactions and may respond to histamine, one of the major mediators of allergy. In order to characterize histamine interactions with PMN, the binding of [3H]pyrilamine was studied. Human PMNs bind [3H]pyrilamine in a specific, saturable, and reversible fashion and demonstrate specificity (H-1 antagonists greater than histamine greater than H-2 antagonists) for the competitive binding agents studied. Human PMNs have a homogeneous population of H-1 receptors of moderate affinity (Kd = 52 nM) in large number (265 X 10(3)/cell) which do not demonstrate cooperativity. Thus PMNs attracted to sites of allergic inflammation have H-1 binding sites which may respond to histamine stimulation.

References

Dec 1, 1975·The Journal of Experimental Medicine·R A ClarkA P Kaplan
Aug 1, 1976·The Journal of Clinical Investigation·G O SolleyA L Schroeter
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E J Goetzl, K F Austen
Sep 1, 1975·The Journal of Experimental Medicine·R K GershonM D Gershon
Jul 1, 1973·The Journal of Allergy and Clinical Immunology·J DolovichJ Bienenstock
May 1, 1973·The Journal of Allergy and Clinical Immunology·P AtkinsB Zweiman
Oct 2, 1980·The New England Journal of Medicine·E J Goetzl

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Citations

Jan 1, 1985·International Journal of Immunopharmacology·T B CasaleM Kaliner
Jan 1, 1996·General Pharmacology·J F KlinkerR Seifert
Aug 30, 2003·International Immunopharmacology·Jun HurJong-Young Kwak
Jun 1, 1989·Journal of Cellular Biochemistry·M Mitsuchashi, D G Payan
Mar 1, 1988·Journal of Cellular Physiology·M Mitsuhashi, D G Payan
Jul 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniela CarlosFernando Q Cunha

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