Histamine H4 receptor mediates chemotaxis of human lung mast cells

European Journal of Pharmacology
Linda J KayPeter T Peachell

Abstract

The diverse effects of histamine are mediated by discrete histamine receptors. The principal repository of histamine in the body is the mast cell. However, the effects of histamine on mast cells, especially those of human origin, have not been fully elucidated. In this study, the expression of histamine receptors in human lung mast cells was evaluated. Moreover, the effects of histamine receptor engagement on both mediator release and chemotaxis were investigated. Mast cells were isolated and purified from human lung tissue. Histamine receptor expression was determined by RT-PCR and q-PCR. Both methods for the detection of histamine receptors were in accordance and human lung mast cells expressed mRNA for histamine H4 and histamine H1 receptors, variably expressed histamine H2 receptor but did not express histamine H3 receptor. The effects of selective histamine receptor agonists on the release of both pre-formed (histamine) and newly-synthesised (cysteinyl-leukotriene, prostaglandin D2) mediators were investigated. None of the agonists tested had any direct effects on mediator release. None of the agonists modulated release stimulated by anti-IgE. Further studies showed that histamine induced migration of mast cells. Chemotaxi...Continue Reading

Citations

Apr 11, 2019·International Journal of Molecular Sciences·Kohei Yamauchi, Masahito Ogasawara
Jun 14, 2019·Frontiers in Immunology·Erika Méndez-Enríquez, Jenny Hallgren
Jul 24, 2020·Cancer Immunology, Immunotherapy : CII·Melisa B NicoudVanina A Medina
Nov 6, 2020·Inflammopharmacology·Madhur D ShastriPhilip M Hansbro
May 2, 2021·Physiological Reports·Anastasia V SudarikovaDaria V Ilatovskaya
Jul 3, 2021·International Journal of Molecular Sciences·Bastian Schirmer, Detlef Neumann
Aug 28, 2021·Biomolecules·Xiaoyuan MaHenry F Vischer

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