Histamine receptor expression in human renal tubules: a comparative pharmacological evaluation

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Eleonora VegliaArianna Carolina Rosa

Abstract

The aim of this study is to evaluate the expression of the histamine receptors, particularly focusing on the H4R in human renal tubules. The ex vivo evaluation was carried on specimens from human renal cortex. Primary and immortalized tubular epithelial cells (TECs) and the HK-2 cell line were used as in vitro models. Cells were pretreated for 10 min with chlorpheniramine maleate 10 μM (H1R antagonist), ranitidine 10 µM (H2R antagonist), GSK189254 1 µM (H3R antagonist) or JNJ7777120 10 µM (H4R antagonist), and then exposed to histamine (3 pM-10 nM) for 30 min. The ex vivo evaluation on specimens from human renal cortex was performed by immunohistochemistry. The expression of histamine receptors on primary and immortalized TECs and the HK-2 cell line was evaluated at both gene (RT-PCR) and protein (immunocytofluorescence) levels. The pharmacological analysis was performed by TR-FRET measurements of second messenger (IP3 and cAMP) production induced by histamine with or without the selective antagonists. Our data revealed the presence of all histamine receptors in human tubules; however, only TECs expressed all the receptors. Indeed, histamine elicited a sigmoid dose-response curve for IP3 production, shifted to the right by chlo...Continue Reading

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Citations

Jun 28, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Alessandro PiniArianna Carolina Rosa
Feb 12, 2019·British Journal of Pharmacology·Cristina GrangeArianna Carolina Rosa
Jan 5, 2019·Clinical Science·Alessandro PiniArianna Carolina Rosa
May 30, 2019·International Journal of Molecular Sciences·Roberta VertaArianna Carolina Rosa
May 2, 2021·Physiological Reports·Anastasia V SudarikovaDaria V Ilatovskaya

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