PMID: 3485790Feb 1, 1986

Histamine reduces Cl- activity in surface epithelial cells of frog gastric mucosa. Suggestive evidence for ionic coupling between surface epithelial and oxyntic cells

Pflügers Archiv : European journal of physiology
S CurciE Frömter

Abstract

Intracellular chloride activity (acCl) and serosal as well as mucosal membrane potentials (Vcs and Vcm) were recorded in surface epithelial cells (SEC) of frog gastric mucosa during the resting state (cimetidine, 10(-4) mol/l) or during stimulation with histamine (10(-4) mol/l). Stimulation leads to a fall in acCl from 18.7 SD +/- 5.9 mmol/l (n = 26) to 13.3 SD +/- 4.9 mmol/l (n = 33). Simultaneously both cell membranes hyperpolarize, Vcs from -56.0 SD +/- 4.8 (n = 42) to -62.8 +/- 7.6 (n = 43) and Vcm from -39.6 SD +/- 5.8 (n = 42) to -47.9 +/- 7.6 (n = 43), so that intracellular chloride remains elevated above electrochemical equilibrium at both cell membranes. Reduction or omission of chloride in the lumen perfusate does not affect acCl, suggesting that the luminal cell membrane is virtually tight for chloride ions. Current induced hyperpolarization of the serosal cell membrane potential which simulates the electrical effects of stimulation, does not affect acCl either; however, inhibition of gastric acid secretion by a benzimidazol derivative which is known to block the H+/K+ ATPase prevents the fall in acCl in response to histamine. The same holds if the experimental solutions are gassed with 25% CO2 which does not interfe...Continue Reading

References

Jan 1, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·S E SjöstrandL Olbe
Dec 30, 1975·Annals of the New York Academy of Sciences·W S Rehm, S S Sanders
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Mar 1, 1985·Pflügers Archiv : European journal of physiology·T Schettino, S Curci
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Citations

Oct 1, 1994·Pflügers Archiv : European journal of physiology·S CurciE Frömter
May 1, 1987·Pflügers Archiv : European journal of physiology·S CurciE Frömter
Apr 14, 1989·Biochimica Et Biophysica Acta·J Alvarez, J García-Sancho
Jan 1, 1990·Journal of Internal Medicine. Supplement·S CurciE Frömter
Nov 14, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·L DebellisS Curci

Related Concepts

Metazoa
Chloride Ion Level
Tagamet
Mesothelium
Gastric Acid
Structure of Pyloric Gland
Ceplene
Resting Potentials
Rana esculenta
In Vitro [Publication Type]

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