Histamine regulates mucin expression through H1 receptor in airway epithelial cells

Acta Oto-laryngologica
Hyoung-Mi KimChae-Seo Rhee

Abstract

The data suggest that histamine up-regulates MUC2 gene regulation and mucin production in airway epithelial cells through histamine 1 receptor (H1R). Histamine appears to play an important role in the early phase of mucin regulation, which might be effectively blocked by an H1R antagonist. Histamine is an important inflammatory mediator during the early phase of allergic response and antihistamine is known to have an ability to reduce mucus secretion in inflamed airways. The goal of the present study was to determine the effects of histamine on MUC2 gene expression and mucin secretion and to investigate the response to histamine 1 receptor (H1R) blocker in NCI-H292 cells and HM3-MUC2 cells. NCI-H292 cells, a human pulmonary mucoepidermoid carcinoma cell line, and HM3-MUC2 cells transfected with MUC2 promoter (-2,864/+19) pGL2 luciferase construct were used in the study. MUC2 mRNA expression was analyzed by RT-PCR for NCI-H292 cells and by luciferase assays for HM3-MUC2 cells. MUC2 protein production was determined by immunoassay and immunofluorescent stain in NCI-H292 cells. Histamine increased MUC2 gene expression in a dose- and time-dependent manner. Peak response was reached at 12 h after histamine administration. MUC2 prote...Continue Reading

References

Nov 1, 1995·Biochemical Society Transactions·I CarlstedtJ R Davies
Jun 19, 1998·Biochimica Et Biophysica Acta·A DohrmanC Basbaum
Feb 17, 2001·American Journal of Respiratory and Critical Care Medicine·C L OrdoñezJ V Fahy
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M TriggianiG Marone
Nov 20, 2001·Current Opinion in Pharmacology·J A Nadel, P R Burgel
Nov 20, 2001·American Journal of Respiratory Cell and Molecular Biology·Y LiK B Adler
Oct 24, 2002·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Lennart GreiffMorgan Andersson
Mar 29, 2003·Current Allergy and Asthma Reports·Maria Susana Repka-Ramirez
Sep 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nancy McNamaraCarol Basbaum
Dec 17, 2005·Current Opinion in Pulmonary Medicine·Esteban J Morcillo, Julio Cortijo
Jul 12, 2007·Pharmacology·Yong Min KimChae-Seo Rhee

❮ Previous
Next ❯

Citations

Dec 21, 2012·Trends in Pharmacological Sciences·Roland SeifertArmin Buschauer
Jul 26, 2015·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·M Nue MøllerP Caye-Thomasen
May 27, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ju Wan KangHyung-Ju Cho
Mar 18, 2015·American Journal of Rhinology & Allergy·Weiwei WangRuitao Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.