Histatin-1 Expression in Human Lacrimal Epithelium

PloS One
Dhara ShahVinay K Aakalu

Abstract

Study of human lacrimal cell biology is limited by poor access to tissue samples, heterogeneous cell composition of tissue and a lack of established lacrimal epithelial markers. In order to further our understanding of lacrimal cell biology, we sought to find a better marker for human lacrimal epithelial cells, compared to what has been reported in the literature. We utilized human Muller's muscle conjunctival resection (MMCR) specimens containing accessory lacrimal gland (ALG) and cadaveric main lacrimal gland (MLG) as sources of lacrimal tissue. Candidate markers were sought using human ALG tissue from MMCR specimens, isolated by laser capture microdissection (LCM). Affymetrix® analysis was performed on total RNA isolated from FFPE samples to profile transcription in ALG. MMCR tissue sections were assessed by immunofluorescence using antibodies for histatin-1, lactoferrin, E-cadherin (E-cad) and alpha-smooth muscle actin (ASMA). Reverse transcriptase polymerase chain reaction (RT-PCR) analysis was performed to analyze the expression of histatin-1, E-cad and lactoferrin from cadaveric MLG. Histatin-1 is expressed in ALG and MLG, localizes to lacrimal epithelium, and to a greater degree than do other putative lacrimal epithelia...Continue Reading

References

Jan 1, 1990·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·C M WilkG O Naumann
Oct 6, 2001·Survey of Ophthalmology·H Brewitt, F Sistani
Jan 10, 2004·International Review of Cytology·Robin R Hodges, Darlene A Dartt
Mar 18, 2004·The Journal of Pharmacy and Pharmacology·Kevin Kavanagh, Susan Dowd
Apr 27, 2004·Experimental Eye Research·Ilene K Gipson
Nov 4, 2004·Ophthalmology·Gary L FillmoreWilliam P Madigan
Dec 6, 2005·Developmental Biology·Irina B GrishinaPaul D Walden
Jun 23, 2006·Infection and Immunity·Marcia M JumblattDonald R Demuth
Sep 14, 2007·Current Eye Research·Ling C HuangAlison M McDermott
Aug 23, 2008·Biochimie·J L Flanagan, M D P Willcox
Jan 23, 2009·Archives of Oral Biology·Melanie CampeseEva J Helmerhorst
Aug 5, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Menno J OudhoffEnno C I Veerman
Mar 23, 2010·Biological Chemistry·Menno J OudhoffEnno C I Veerman
Dec 2, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Mechthild StoeckelhuberMarco R Kesting
Jan 19, 2012·PloS One·Shubha TiwariGeeta K Vemuganti
Mar 20, 2012·Investigative Ophthalmology & Visual Science·Marie A ShatosDarlene A Dartt
Sep 8, 2012·Investigative Ophthalmology & Visual Science·John L UbelsMark P Hatton
Nov 3, 2012·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Yanfang SiYannian Hui
Oct 3, 2013·Nature Communications·Masatoshi HirayamaTakashi Tsuji
Feb 15, 2014·Saudi Journal of Ophthalmology : Official Journal of the Saudi Ophthalmological Society·Shubha TiwariGeeta K Vemuganti

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Citations

Dec 13, 2017·Oral Diseases·P TorresV A Torres
Jun 28, 2018·Current Eye Research·Marko OydanichPenny A Asbell
May 26, 2017·PloS One·Dhara ShahVinay Kumar Aakalu
Jan 13, 2017·PloS One·Vinay Kumar AakaluSubramanian Krishnakumar
Jan 17, 2021·International Journal of Molecular Sciences·Youssof EshacVinay Kumar Aakalu
Feb 9, 2021·Journal of Tissue Viability·Li PanQiong Gao
Apr 30, 2020·Molecular Therapy. Methods & Clinical Development·Dhara ShahVinay Kumar Aakalu
Aug 8, 2021·International Journal of Molecular Sciences·Sang Min LeeVinay Kumar Aakalu

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Methods Mentioned

BETA
PCR
Chip
dissection

Software Mentioned

ALG

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