Abstract
The human leukemic cell line KU-812-F is known to differentiate into mature basophil-like cells under serum-free culture conditions. In the present study, the activity of histidine decarboxylase (HDC), a histamine-forming enzyme, in KU-812-F cells was found to be high, ranging from 10 to 57 pmol/min/mg protein. The great variation in HDC activity appeared to be due to different percentages and degrees of maturity of basophil-like cells during differentiation of this cell line. The enzyme was inhibited by alpha-fluoromethylhistidine but not by carbidopa, was unable to form dopamine from L-3,4-dihydroxyphenylalanine, and had a Km value for histidine of 0.27 mM, indicating that it was HDC and not aromatic amino acid decarboxylase. The HDC activity increased 1.8-fold when the cells were stimulated by phorbol myristate acetate, which is known to activate protein kinase C, and this increase was blocked by staurosporine, a potent inhibitor of protein kinase C.
References
Apr 1, 1979·Biochemical Pharmacology·T WatanabeH Wada
Nov 15, 1979·Analytical Biochemistry·T NagatsuT Kato
Aug 31, 1978·Nature·J KollonitschS D Aster
Oct 22, 1975·Biochimica Et Biophysica Acta·L HammarS Hjertén
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A ShimizuP Leder
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Mar 30, 1988·Biochemical and Biophysical Research Communications·K MaeyamaT Watanabe
Mar 13, 1986·Biochemical and Biophysical Research Communications·T TamaokiF Tomita
Nov 26, 1986·Biochemical and Biophysical Research Communications·M Ando-YamamotoH Wada
May 1, 1987·The Tohoku Journal of Experimental Medicine·Y ShindohT Takishima
Jan 1, 1987·Leukemia Research·S A FischkoffJ Hakimi
Mar 1, 1986·The Biochemical Journal·S A Martin, J O Bishop
Jul 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·N YabeM Takada
May 31, 1985·Biochemical and Biophysical Research Communications·K Kawaguchi-NagataK Shoji
Oct 1, 1985·Biochemical Pharmacology·A S TungH J Zweerink
Nov 8, 1985·Journal of Chromatography·A YamatodaniT Watanabe
Apr 19, 1984·Nature·Y Nishizuka
Feb 1, 1984·Agents and Actions·M YamadaH Wada
Apr 15, 1982·Biochemical Pharmacology·Y Endo
Aug 1, 1982·The Biochemical Journal·A Savany, L Cronenberger
May 15, 1981·Biochemical and Biophysical Research Communications·T WatanabeY Kitamura
Oct 1, 1980·Biochimica Et Biophysica Acta·M YamadaH Wada
Aug 1, 1980·Journal of Neurochemistry·G BarbinY Agid
Jun 1, 1960·The American Journal of Physiology·R W SCHAYER
Citations
Oct 17, 2013·Amino Acids·R Castro-OropezaF Sánchez-Jiménez
Jul 31, 1999·Experimental Hematology·M DyE Schneider
Oct 15, 1992·European Journal of Biochemistry·R Mamune-SatoT Takishima
Dec 7, 2002·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Takehiko Watanabe, Hiroshi Ohtsu
Jan 6, 2009·The Journal of Allergy and Clinical Immunology·Laura MaintzNatalija Novak
Aug 22, 2000·The Journal of Investigative Dermatology·M Haak-FrendschoA Falus
Jun 14, 1996·The Journal of Biological Chemistry·Z ZhangT C Wang
Dec 13, 2017·Theoretical Biology & Medical Modelling·Janet BestMichael Reed
Dec 22, 1995·The Journal of Biological Chemistry·K YatsunamiT Higuchi
Aug 12, 1998·The American Journal of Physiology·C A ZahnowD E Millhorn