PMID: 7543025Jul 28, 1995Paper

Histidine phosphorylation of P-selectin upon stimulation of human platelets: a novel pathway for activation-dependent signal transduction

Cell
C S CrovelloB Furie

Abstract

Transient phosphorylation of histidine characterizes the two-component systems in prokaryotes that control important physiological functions, but analogous events have not been implicated in signal transduction in mammalian cells. To explore histidine phosphorylation during activation of human cells, stimulated platelets were analyzed for the formation of protein phosphohistidine in a model system employing P-selectin. P-selectin, a leukocyte adhesion molecule, undergoes rapid phosphorylation and selective dephosphorylation of tyrosine, serine, and threonine. We now establish that phosphorylation following platelet activation with thrombin or collagen generates phosphohistidine at histidines on the cytoplasmic tail of P-selectin. With thrombin stimulation, the kinetics of phosphohistidine appearance and disappearance of P-selectin are very rapid. Platelets exhibit a novel ligand-induced signaling pathway to generate phosphohistidine. These results provide direct biochemical evidence for the induction of rapid and reversible histidine phosphorylation in mammalian cells upon cell activation and represent a novel paradigm for mammalian cell signaling.

References

Nov 1, 1977·Biochemistry·C C ChenR A Smith
Aug 19, 1991·FEBS Letters·U B RasmussenE Van Obberghen-Schilling
Jan 1, 1991·Annual Review of Biochemistry·R B BourretM I Simon
Mar 29, 1990·Nature·J B StockJ M Mottonen
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·A CeliB Furie
Nov 1, 1994·Trends in Biochemical Sciences·R V SwansonM I Simon
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·S R Coughlin

❮ Previous
Next ❯

Citations

Jul 1, 1997·Protein Science : a Publication of the Protein Society·K F MedzihradszkyC W Turck
Nov 15, 2006·Amino Acids·P V AttwoodP G Besant
Apr 24, 2009·Molecular and Cellular Biochemistry·Paul G Besant, Paul V Attwood
Jun 22, 1999·Molecular and Biochemical Parasitology·I Hunger-GlaserT Seebeck
Jun 22, 1999·Chemistry & Biology·M C Pirrung
Jan 18, 2003·The International Journal of Biochemistry & Cell Biology·Paul G BesantPaul V Attwood
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·P G Besant, P V Attwood
Feb 22, 2002·European Journal of Biochemistry·Susanne Klumpp, Josef Krieglstein
Nov 5, 1999·Protein Science : a Publication of the Protein Society·M LaskerC W Turck
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Robert D. Smith, John C. Walker
Dec 29, 1998·Analytical Biochemistry·P G Besant, P V Attwood
Aug 19, 2007·Coronary Artery Disease·Ayse Saatci YasarIrfan Sabah
Jul 12, 2002·American Journal of Hematology·David GurneyAndrew D Blann
Apr 17, 2012·Organic & Biomolecular Chemistry·Tom E McAllister, Michael E Webb
Aug 30, 2011·Bioorganic Chemistry·Phillip A Schwartz, Brion W Murray
May 24, 2011·Thrombosis Research·Jean-François ThéorêtYahye Merhi
Dec 7, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Susanne KlumppJosef Krieglstein
Oct 29, 2003·Cytometry. Part B, Clinical Cytometry·Angel V DelgadoAnthony E Pusateri
Jun 6, 2003·Biochemical and Biophysical Research Communications·Susanne KlumppJosef Krieglstein
Oct 1, 2005·Biochimica Et Biophysica Acta·Susanne Klumpp, Josef Krieglstein
Jul 26, 2005·Biochemical and Biophysical Research Communications·Anette MäurerSusanne Klumpp
Oct 27, 2012·Journal of Proteomics·James D R KnightAnne-Claude Gingras
Jul 19, 2013·Biochemical Society Transactions·Tom E McAllisterMichael E Webb
Mar 28, 2012·Biochimica Et Biophysica Acta·Paul V Attwood
Dec 12, 1996·Biochimica Et Biophysica Acta·L A Pinna, M Ruzzene
Nov 26, 2010·Chemical Communications : Chem Comm·Tom E McAllisterMichael E Webb
Jul 18, 2000·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·R AltmanA Scazziota
Dec 5, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Mehul V MakwanaRichard Fw Jackson
Oct 3, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Imran Khan, Patricia S Steeg
Jun 23, 1998·The Journal of Biological Chemistry·S H SoderlingJ A Beavo
Aug 25, 2000·The Journal of Biological Chemistry·R MuimoA Mehta
Jan 29, 1999·Physiological Reviews·D Vestweber, J E Blanks
Sep 10, 2003·American Journal of Physiology. Endocrinology and Metabolism·Anjaneyulu Kowluru

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.