Histidine-rich protein Hpn from Helicobacter pylori forms amyloid-like fibrils in vitro and inhibits the proliferation of gastric epithelial AGS cells

Biochimica Et Biophysica Acta
Ruiguang GeHongzhe Sun

Abstract

Helicobacter pylori causes various gastric diseases, such as gastritis, peptic ulcerations, gastric cancer and mucosa-associated lymphoid tissue lymphoma. Hpn is a histidine-rich protein abundant in this bacterium and forms oligomers in physiologically relevant conditions. In this present study, Hpn oligomers were found to develop amyloid-like fibrils as confirmed by negative stain transition electron microscopy, thioflavin T and Congo red binding assays. The amyloid-like fibrils of Hpn inhibit the proliferation of gastric epithelial AGS cells through cell cycle arrest in the G2/M phase, which may be closely related to the disruption of mitochondrial bioenergetics as reflected by the significant depletion of intracellular ATP levels and the mitochondrial membrane potential. The collective data presented here shed some light on the pathologic mechanisms of H. pylori infections.

References

Aug 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W E KlunkD J Abraham
Aug 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W E KlunkD J Abraham
May 5, 1994·The New England Journal of Medicine·J ParsonnetG D Friedman
Apr 1, 1996·Molecular Microbiology·T L Cover
Oct 8, 1999·Biochemical and Biophysical Research Communications·J Y SukW J Park
Mar 10, 2001·Nature·M FändrichC M Dobson
Jun 26, 2001·The Journal of Biological Chemistry·Y YoshiikeA Takashima
Jan 5, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ehud Gazit
Sep 11, 2003·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·John B Ancsin
Jan 16, 2004·The Journal of Biological Chemistry·Sangita C SinhaJanet L Smith
Sep 17, 2005·Nature Reviews. Molecular Cell Biology·Christopher A Ross, Michelle A Poirier
Jun 8, 2006·Annual Review of Biochemistry·Fabrizio Chiti, Christopher M Dobson
Aug 31, 2006·Journal of the American Chemical Society·Ruiguang GeHongzhe Sun
Sep 20, 2006·Accounts of Chemical Research·Tadato BanYuji Goto
Jan 6, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Robert J MaierSusmitha Seshadri
Apr 7, 2007·Trends in Biochemical Sciences·Douglas M FowlerJeffery W Kelly
May 16, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ruiguang GeHongzhe Sun
Apr 16, 2008·Cellular Microbiology·Steffen Backert, Matthias Selbach
Feb 17, 2009·Biophysical Journal·Christian Beyschau AndersenChristian Rischel
Jun 25, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Elena ElimovaJohn B Ancsin
Jul 29, 2010·Physiological Reviews·Richard M PeekKeith T Wilson
Sep 11, 2010·Cognitive and Behavioral Neurology : Official Journal of the Society for Behavioral and Cognitive Neurology·Jannis KountourasIoannis Venizelos

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Citations

Dec 24, 2013·World Journal of Gastroenterology : WJG·Rui-Guang GeXue-Song Sun
Jul 8, 2015·Therapeutic Delivery·Adeola O Adebisi, Barbara R Conway
Nov 23, 2013·European Journal of Microbiology & Immunology·Kerstin A HeylStefan Bereswill
Oct 4, 2014·World Journal of Gastroenterology : WJG·Traci L Testerman, James Morris
Aug 9, 2013·Metallomics : Integrated Biometal Science·Tianfan ChengHongzhe Sun
Mar 25, 2021·Scientific Reports·Stéphane L Benoit, Robert J Maier

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