Histidylated cationic polyorganophosphazene/DNA self-assembled nanoparticles for gene delivery

International Journal of Pharmaceutics
Yongxin YangYaping Li

Abstract

Cationic polyorganophosphazene has shown the ability to deliver gene. To obtain more efficient transfection, His(Boc)-OMe bearing histidine moiety was introduced to synthesize a new derivative of cationic polyphosphazenes with another side group of 2-dimethylaminoethylamine (DMAEA). The poly(DMAEA/His(Boc)-OMe)phosphazene (PDHP) and DNA could self-assemble into nanoparticles with a size around 110 nm and zeta potential of +15 mV at the PDHP/DNA ratio of 10:1 (w/w). The maximum transfection efficiency of PDHP/DNA self-assembled nanoparticles (PHSNs) against 293 T cells was much higher than that of poly(di-2-dimethylaminoethylamine) phosphazenes (PDAP)/DNA self-assembled nanoparticles (PASNs) and PEI 25/DNA self-assembled nanoparticles (PESNs) at the polymer/DNA ratio of 10:1, but the cytotoxicity of PDHP assayed by MTT was much lower than that of PDAP and PEI 25. These results suggested that PDHP could be a good candidate with high transfection efficiency and low cytotoxicity for gene delivery.

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Citations

Feb 6, 2010·Journal of Materials Science. Materials in Medicine·Jong-Yuh CherngMin-Da Shau
Apr 1, 2011·Therapeutic Delivery·Joel C SunshineJordan J Green
Apr 28, 2010·International Journal of Pharmaceutics·Chao-Hsien LinMin-Da Shau
Feb 17, 2009·Acta Biomaterialia·Xuetao ShiDong-An Wang
Jul 26, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Zahra SalmasiMohammad Ramezani
Jul 20, 2011·Journal of Polymer Science. Part B, Polymer Physics·Bret D UleryCato T Laurencin
Feb 7, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhipeng NiLison Teng
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Mar 28, 2019·Biomaterials Research·Y K Sung, S W Kim
Aug 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Tatiana V BurovaAlexei R Khokhlov
Jun 29, 2018·Biomacromolecules·Alexander K AndrianovThomas R Fuerst

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