Histo-molecular differentiation of renal cancer subtypes by mass spectrometry imaging and rapid proteome profiling of formalin-fixed paraffin-embedded tumor tissue sections

Oncotarget
Uwe MögingerOle N. Jensen

Abstract

Pathology differentiation of renal cancer types is challenging due to tissue similarities or overlapping histological features of various tumor (sub) types. As assessment is often manually conducted outcomes can be prone to human error and therefore require high-level expertise and experience. Mass spectrometry can provide detailed histo-molecular information on tissue and is becoming increasingly popular in clinical settings. Spatially resolving technologies such as mass spectrometry imaging and quantitative microproteomics profiling in combination with machine learning approaches provide promising tools for automated tumor classification of clinical tissue sections. In this proof of concept study we used MALDI-MS imaging (MSI) and rapid LC-MS/MS-based microproteomics technologies (15 min/sample) to analyze formalin-fixed paraffin embedded (FFPE) tissue sections and classify renal oncocytoma (RO, n = 11), clear cell renal cell carcinoma (ccRCC, n = 12) and chromophobe renal cell carcinoma (ChRCC, n = 5). Both methods were able to distinguish ccRCC, RO and ChRCC in cross-validation experiments. MSI correctly classified 87% of the patients whereas the rapid LC-MS/MS-based microproteomics approach correctly classified 100% of the...Continue Reading

References

Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·W ThoenesH J Rumpelt
Jan 15, 1999·Journal of the American Society for Mass Spectrometry·M StoeckliR M Caprioli
Sep 4, 2003·Genome Research·Paul D ThomasApurva Narechania
Dec 5, 2006·Genome Biology·Alexander Schmidt, Ruedi Aebersold
Sep 12, 2009·Angewandte Chemie·Karl-Christian SchäferZoltán Takáts
Feb 10, 2010·Journal of Proteome Research·Stacey R OppenheimerRichard M Caprioli
Aug 25, 2010·Biochimica Et Biophysica Acta·Giuseppe GasparreAnna Maria Porcelli
Nov 11, 2010·Methods in Molecular Biology·Andreas RömppBernhard Spengler
Jan 10, 2012·Journal of Proteome Research·Stephan MedingAxel Walch
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·J K EngJ R Yates
Nov 12, 2014·BMJ : British Medical Journal·Eric JonaschW Kimryn Rathmell
Jul 15, 2015·Annual Review of Analytical Chemistry·Shannon Eliuk, Alexander Makarov
Jul 18, 2015·Science·M I Jordan, T M Mitchell
Jul 18, 2015·Radiologia brasileira·Valdair F Muglia, Adilson Prando
Jun 28, 2016·Nature Methods·Stefka TyanovaJürgen Cox
Jun 29, 2016·Nature Communications·Yunee KimThomas Kislinger
Jan 27, 2017·Molecular & Cellular Proteomics : MCP·Hannes HinneburgDaniel Kolarich
Apr 21, 2017·Journal of Clinical Pathology·Mark KriegsmannJörg Kriegsmann
May 20, 2017·Methods in Molecular Biology·Maxence WisztorskiIsabelle Fournier
Sep 9, 2017·Future Science OA·Liam M HeaneyToru Suzuki
Nov 11, 2017·Bioinformatics·Jens BehrmannPeter Maaß
Dec 12, 2017·Rapid Communications in Mass Spectrometry : RCM·Daniel J RyanJeffrey M Spraggins
Apr 16, 2018·Methods : a Companion to Methods in Enzymology·Nadine E MasciniRon M A Heeren
May 23, 2018·Nature Methods·Shane R EllisRon M A Heeren
Aug 3, 2018·Analytical Chemistry·Khalil MallahIsabelle Fournier
Aug 9, 2018·Clinical Proteomics·Vanessa DrendelOliver Schilling

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Methods Mentioned

BETA
electron microscopy
PCA
biopsies
proteome profiling
xenograft

Software Mentioned

EvoSep
String DB
Cardinal
R MSI package
StringDB
Panther DB
ClustVis
imzML
Panther
PLS

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