PMID: 2499620Jul 1, 1989Paper

Histochemical analysis of the chemical structure of blood group-related carbohydrate chains in serous cells of human submandibular glands using lectin staining and glycosidase digestion

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
N ItoT Hirota

Abstract

Using lectin staining methods in combination with exo- and endo-glycosidase digestion procedures, we analyzed the chemical structure of different types of blood group-related substances in serous cells of formalin-fixed, paraffin-embedded human submandibular glands. Serous cells produced only H antigen; A and B antigens were not present, and the expression of H antigen is dependent on the secretor status of the tissue donor. Although reactivity with Ulex europaeus agglutinin I (UEA-I) was not markedly reduced by alpha-L-fucosidase digestion, an affinity for peanut agglutinin (PNA) was seen after fucosidase digestion in the cells from secretors. In those from nonsecretors, no PNA reactivity appeared after enzyme digestion. On the other hand, sialidase digestion elicited PNA reactivity in serous cells irrespective of the donor's secretor status. PNA reactivity observed after fucosidase or sialidase digestion was susceptible to endo-alpha-N-acetylgalactosaminidase (endo-GalNAc-dase) digestion. SBA reactivity in UEA-I-negative cells from secretors, or in cells from fetuses and newborn infants, was markedly reduced by beta-galactosidase digestion. After galactosidase digestion, reactivity with Griffonia simplicifolia agglutinin II (...Continue Reading

Citations

Jan 1, 1992·Progress in Histochemistry and Cytochemistry·N Ito, T Hirota
Jun 22, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y LiuH Kimura
Dec 24, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Y H LiuH Kimura
Aug 1, 1994·The Anatomical Record·L M PastorJ A Marin
Dec 19, 2019·Molecular & Cellular Proteomics : MCP·Brian B Haab, Zachary Klamer
Feb 1, 1994·Journal of Dental Research·M S Lantini, M Cossu
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Brian B Haab, Zachary Klamer
Mar 16, 2018·ACS Chemical Biology·John B McArthurXi Chen

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