Histochemical and biochemical studies of carbonic anhydrase activity in the opercular epithelium of the euryhaline teleost, Fundulus heteroclitus

The American Journal of Anatomy
E R Lacy

Abstract

Carbonic anhydrase (CAH) activity was biochemically measured and histochemically localized (at both the light and electron microscope levels) in isolated opercular membranes from teleost fish, Fundulus heteroclitus, adapted to freshwater (FW), seawater (SW), and double-strength seawater (2 x SW). The normal morphology of this membrane showed that its epithelial portion consisted of five cell types: (1) chloride cells, which have been previously implicated as responsible for the active chloride transport across the epithelium; (2) mucous cells; (3) pavement cells, which formed the major portion of the free epithelial surface; (4) supportive cells, which had an abundance of intermediate (10 nm)-type filaments suggesting a structural role for these cells; and (5) vesicular cells, which were characterized by various types of membrane-bound vesicles, including lysosomes, and numerous free ribosomes. Vesicular cells may be stem cells and/or endocrine cells. Hansson's histochemical method for CAH revealed cobalt sulfide reaction product confined to the following structures in fish from each environment: (1) chloride cells: throughout the cytoplasm and some nuclear staining; (2) mucous cells: throughout the cytoplasm, some nuclear stai...Continue Reading

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May 1, 1987·Fish Physiology and Biochemistry·D J Randall, P A Wright
Dec 31, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Chris M Wood, Martin Grosell
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Apr 1, 1983·The Journal of Experimental Zoology·E R Lacy
Apr 1, 1988·Journal of Morphology·Pung-Pung Hwang
Mar 24, 2004·The Journal of Experimental Biology·Leonard B Kirschner

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