Histochemical classification of neck and limb muscle fibers in a turtle, Pseudemys scripta: a study using microphotometry and cluster analysis techniques

Journal of Morphology
R J CallisterE H Peterson

Abstract

We have attempted to develop an objective, semiquantitative classification of fiber types in turtle neck and limb muscle using microphotometry and multivariate statistical techniques. We first stained serial sections for myosin adenosine triphosphatase (ATPase) (with acid and alkaline preincubation and without preincubation), NADH-diaphorase, and two glycolysis-associated markers, alpha-glycerophosphate dehydrogenase (alpha-GPDH) and glycogen phosphorylase A (GPA). This allowed us to characterize individual muscle fibers in terms of their contraction speed and metabolic properties. Next we used microphotometry to measure the optical density of the reaction product in each fiber, and we subjected the resulting optical density matrix to cluster and discriminant function analyses in order to assign fibers to groups (fiber types) and to determine which stains contribute most to the distinction between groups. As a control, we processed a well characterized mammalian muscle (rat sternomastoid) simultaneously. Our results suggest that both neck and limb muscle in Pseudemys can best be described as falling into three groups: 1) slow oxidative (SO) fibers; 2) fast oxidative glycolytic (FOG) fibers, with relatively high oxidative and gl...Continue Reading

References

Sep 1, 1979·The American Journal of Anatomy·A F Dulhunty, M Dlutowski
Jan 17, 1986·Science·P W Hochachka
Mar 22, 1974·Annals of the New York Academy of Sciences·M H Brooke, K K Kaiser
Aug 1, 1970·Experimental Neurology·L Guth, F J Samaha
Sep 1, 1970·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M H Brooke, K K Kaiser
Dec 1, 1968·The Journal of Physiology·U Proske, P Vaughan
Apr 1, 1966·The Journal of Physiology·L Levine
Jul 1, 1982·The Anatomical Record·G S Throckmorton, C W Saubert
Jan 1, 1980·Histochemistry·D PetteP M Nemeth
Jan 1, 1961·The Journal of Biophysical and Biochemical Cytology·A B NOVIKOFFJ DRUCKER
Jul 1, 1960·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L W WATTENBERG, J L LEONG
Mar 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T OGATA, M MORI

Related Concepts

Hindlimb
Cytochemistry
Muscle
Skeletal Muscle Structure of Neck
Photometry
Staining and Labeling
Turtle

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