PMID: 6981265May 1, 1982Paper

Histochemical demonstration of an ATP-dependent Ca2+-pump in bullfrog myocardial cells

Zeitschrift Für Naturforschung. Section C: Biosciences
R MeyerH G Haas

Abstract

In the present investigation different finestructural and histochemical procedures were employed to demonstrate normal morphology and Ca2+-transport mechanism in bullfrog atrial myocardium. For normal morphology specimens were fixed in 1% OsO4, 2.5% glutaraldehyde or liquid propane at -185 degrees C before they were prepared for conventional embedding and freeze-etching, respectively. Special interest was focussed on the caveolae system, which is composed of single, spherical membrane invaginations (diameter, 85 nm), randomly distributed at the entire cell periphery. The caveolae enlarge the cell surface by 24.5% and occupy 8.5% of the cellular volume. The caveolae membrane contains a few intramembraneous particles with a diameter of 8.4 nm comparable to the size of ATPases as found in other cells. For histochemistry the specimens were first stabilized by treatment with 50% glycerol, 0.025% glutaraldehyde or 0.15% formaldehyde and then incubated in a medium containing 4 mM CaCl2, 4 mM EGTA, 5 mM MgCl2, 5 mM K2C2O4, 5 mM ATP and 20 mM histidine at pH 7.0. This incubation always succeeded in the formation of electron-dense deposits with elliptical shape, measuring 100-200 nm in length and 10-30 nm in diameter. According to X-ray ...Continue Reading

Citations

Dec 1, 1983·Pflügers Archiv : European journal of physiology·H G HaasW Stockem
Jan 1, 1986·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·R VolkmannL E Ericson
Jan 1, 1988·Comparative Biochemistry and Physiology. A, Comparative Physiology·R Volkmann
Mar 28, 2009·Cell Calcium·Biswaranjan Pani, Brij B Singh

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