PMID: 7540049Jan 1, 1995Paper

Histochemical demonstration of DNA double strand breaks by in situ 3'-tailing reaction in apoptotic endometrium

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
T NakamuraM Hotchi

Abstract

A new histochemical technique, called in situ 3'-tailing reaction (ISTR), to detect DNA double strand breaks (DSB) was developed and applied to tissue sections of apoptotic endometrium. To demonstrate DSB, biotin-labeled and unlabeled dATPs with terminal deoxynucleotidyl transferase (TdT) were added to the many 3-hydroxyl termini of DNA fragments generated in the apoptotic cells. For an efficient 3'-end labeling, it was necessary to treat the sections with lambda-exonuclease (lambdaEx) prior to the TdT reaction to generate 3'-protruding ends. The lambdaEx-TdT reaction specifically labeled nuclear fragments in the apoptotic cells in paraformaldehyde fixed frozen sections. In paraffin sections, pretreatment with proteinase K was effective for 3'-tailing reaction. ISTR should be a useful tool for detecting dying cells in both physiological and pathological states.

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Citations

Aug 1, 1996·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D VinatierD Subtil
Aug 21, 2013·Reproductive Biomedicine Online·Sheena E M LewisParviz Gharagozloo
Jul 20, 2002·BMC Biotechnology·Shumo LiuAlbert Libchaber
Jul 27, 1999·Pathology International·T Nakamura
Mar 4, 1999·Histopathology·C J StewartM A Farquharson
May 11, 1999·Journal of Cutaneous Pathology·T Nakamura
Aug 20, 2010·Systems Biology in Reproductive Medicine·Monica MuratoriElisabetta Baldi

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