PMID: 90411Jan 1, 1979Paper

Histochemical demonstration of enzyme activities in plastic and paraffin embedded tissue sections

Stain Technology
S HiguchiB H Schofield

Abstract

Histochemical staining for enzymes is usually performed on frozen sections. This report lists the longer incubation times required to demonstrate esterase, acid phosphatase, beta-galactosidase, and cytochrome oxidase in plastic embedded and ruotine paraffin embedded tissues. The sections embedded in plastic, i.e. water soluble methacrylate (Polyscience's JB-4) and cut at 2 micrometers, were far superior to frozen sections and paraffin embedded sections both in tissue detail and in the localization of the histochemical reaction product.

References

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Citations

Jan 1, 1980·Histochemistry·F M Eggert, J P Germain
Aug 15, 1983·Experientia·N Ihara
Dec 1, 1984·Calcified Tissue International·H A SissonsG Marotti
Sep 27, 2003·European Journal of Pharmacology·Fumiaki ItohHiroshi Kogo
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Nov 3, 2012·Plant Science : an International Journal of Experimental Plant Biology·Irene Serrano, Adela Olmedilla
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Related Concepts

Acid Phosphatase
Lactogest
Cytochrome c Oxidase Subunit VIa
Esterases
Galactosidase
Cytochemistry
Macrophage
Mycobacterium bovis
Parafilm
Plastics

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