Histochemical demonstration of nitric oxide in herniated lumbar discs. A clinical and animal model study

Spine
H HashizumeT Tamaki

Abstract

This study was designed to localize the cells that produce nitric oxide in a lumbar disc herniation by histochemical method, including in situ hybridization. To clarify which cells in herniated lumbar discs produce nitric oxide. It was reported that herniated lumbar intervertebral disc specimens in culture are capable of producing nitric oxide. Surgical specimens from lumbar disc herniation were examined to determine nitric oxide synthase histologically using nicotinamide adenine dinucleotide phosphate diaphorase histochemistry. Allografts of intervertebral disc materials were placed on the epidural space at L6 level in the rat. Nitric oxide synthase was examined in the applied tissues using nicotinamide adenine dinucleotide phosphate diaphorase histochemistry and in situ hybridization histochemistry. Nicotinamide adenine dinucleotide phosphate diaphorase (nitric oxide synthase) positive cells were observed in 2 (40%) of 5 herniated disc materials in patients. The positive cells were mainly in granulation tissue around intervertebral disc materials. In animal models, nitric oxide synthase-positive cells were observed in all specimens at 1 and 2 weeks postoperatively. Newly formed vessels and small round cells in granulation tis...Continue Reading

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Citations

Aug 31, 2001·Journal of Cellular Biochemistry·M K ElfervigA J Banes
Dec 29, 2000·Radiologic Clinics of North America·I W McCall
Jun 15, 2006·The Clinical Journal of Pain·YiLi Zhou, Salahadin Abdi
Jan 13, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M KawakamiT Tamaki
May 18, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M KawakamiT Kihira
Jun 4, 2010·European Journal of Anaesthesiology·Oonagh T HickeyGeorge D Shorten
Mar 5, 2003·Clinical Orthopaedics and Related Research·Tsukasa OnozawaXin Feng
Nov 19, 2002·Spine·Hiroshi MiyamotoKosaku Mizuno
Jul 25, 2000·Clinical Orthopaedics and Related Research·M KawakamiM Shinozaki
Oct 31, 2001·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·F C VinasS Rengachary

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